Burci Lígia Moura, da Silva Cristiane Bezerra, Rondon Josimara Nolasco, da Silva Luisa Mota, de Andrade Sérgio Faloni, Miguel Obdulio Gomes, de Fátima Gaspari Dias Josiane, Miguel Marilis Dallarmi
Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF), Universidade Federal do Paraná (UFPR), Centro Politécnico, Jardim das Américas, 81531-970, Curitiba, PR, Brazil.
Programa de Pós-Graduação em Ciências Ambientais, Universidade Católica Dom Bosco (UCDB), Av. Tamandaré, 6000, Jardim Seminário, 79117-900, Campo Grande, MS, Brazil.
Toxicol Rep. 2018 Feb 23;6:1304-1308. doi: 10.1016/j.toxrep.2018.02.006. eCollection 2019.
Plants and their parts are a part of life in many Brazilian communities, as observed in the jackfruit. The jackfruit seeds are consumed, usually, as roasted, boiled, steamed, and are eaten as a snack.
The present study was carried out to identify the seeds toxicity and cytotoxic activity.
The extracts were tested in toxicity assays like, brine shrimp lethality assay, hemolysis assay, and effect of seed extracts on T47D, TH29 and B16F10 cancer cell lines, and in acute and subchronic toxicity in mice.
seed presents no toxic effects in brine shrimp, no hemolytic activity, and was effective in cancer cell lines like T47D, TH29 and B16F10. IC50 obtained from extracts was 46.67 μg/ml of chloroform extract in T47D cells, 23.42 μg/ml of ethanolic extract in HT29 cells, and 74.31 μg/ml of ethyl acetic extract in B16F10 cells. Ethanolic extract presented zero lethality index and was able to reduce the level of glycemia in females (32.3%) in the subchronic test.
With this results we can conclude that seeds presents no toxicity, and is very effective in determinated cancer cell lines, requiring further studies to validate their use as active natural product against cancer cells.
正如在菠萝蜜中所观察到的那样，植物及其部分是许多巴西社区生活的一部分。菠萝蜜种子通常经过烘烤、水煮、蒸煮后食用，作为一种小吃。
开展本研究以确定种子的毒性和细胞毒性活性。
对提取物进行了多种毒性试验，如卤虫致死率试验、溶血试验，以及种子提取物对T47D、TH29和B16F10癌细胞系的影响，并对小鼠进行急性和亚慢性毒性试验。
种子对卤虫无毒性作用，无溶血活性，对T47D、TH29和B16F10等癌细胞系有效。从提取物中获得的半数抑制浓度（IC50）在T47D细胞中，氯仿提取物为46.67μg/ml；在HT29细胞中，乙醇提取物为23.42μg/ml；在B16F10细胞中，乙酸乙酯提取物为74.31μg/ml。乙醇提取物的致死指数为零，在亚慢性试验中能够降低雌性小鼠的血糖水平（32.3%）。
根据这些结果我们可以得出结论，种子没有毒性，对特定癌细胞系非常有效，需要进一步研究以验证其作为抗癌活性天然产物的用途。
