Laboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de Tepic, Av. Tecnológico 2595, C.P. 63175 Tepic, Nayarit, Mexico.
Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, C.P. 8300 Hermosillo, Sonora, Mexico.
Food Chem. 2015 May 15;175:409-16. doi: 10.1016/j.foodchem.2014.11.122. Epub 2014 Nov 28.
The present focused on the study of the antimutagenic and antiproliferative potential of pulp Jackfruit (Artocarpus heterophyllus Lam) extract, using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line M12.C3.F6 (murine B-cell lymphoma), respectively. Jackfruit pulp extract was sequentially fractionated by chromatography (RP-HPLC) and each fraction was tested for antimutagenic and antiproliferative activities. The organic extracts obtained from Jackfruit pulp reduced the number of revertants caused by aflatoxin B1 (AFB1) and proliferation of cells M12.C3.F6; a dose-response relationship was showed. Sequential RP-HPLC fractionation of the active extracts produced both antimutagenic and/or antiproliferative fractions. These results suggested that the Jackfruit contained compounds with chemoprotective properties to reduce the mutagenicity of AFB1, also proliferation of a cancer cell line.
本研究聚焦于研究菠萝蜜(Artocarpus heterophyllus Lam)果肉提取物的抗突变和抗增殖潜力,使用分别带有代谢激活(S9)的鼠伤寒沙门氏菌测试菌株 TA98 和 TA100 以及癌细胞系 M12.C3.F6(鼠 B 细胞淋巴瘤)作为测试对象。菠萝蜜果肉提取物通过色谱(RP-HPLC)进行顺序分段,每个分段都进行了抗突变和抗增殖活性测试。从菠萝蜜果肉中获得的有机提取物减少了黄曲霉毒素 B1(AFB1)引起的回复突变数和 M12.C3.F6 细胞的增殖;表现出剂量反应关系。活性提取物的顺序 RP-HPLC 分段产生了具有抗突变和/或抗增殖作用的分段。这些结果表明,菠萝蜜中含有具有化学保护特性的化合物,可以降低 AFB1 的致突变性,同时也可以抑制癌细胞系的增殖。