Serum IgG Anti- Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors.

infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti- antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti- antibody concentrations correlate Nested PCR results in blood donors. 750 blood donors were enrolled. IgM and IgG anti- antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115 bp) of the gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the -test or Mann-Whitney according to the normal distribution (-value ≤ 0.05). 361 (48.1%) blood donors presented positive serology as follow: IgM/IgG: 5 (0.6%); IgM/IgG: 21 (2.8%); IgM/IgG: 335 (44.7%) and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti- antibody concentrations and the Nested PCR results. In conclusion, our data show that variations in the serum IgG anti- antibody concentrations do not correlate parasitemia detected by Nested PCR in chronically infected healthy blood donors.