Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto, São Paulo, Brazil.
Blood Bank São José do Rio Preto, Fundação Faculdade Regional de Medicina, São Paulo, Brazil.
Front Cell Infect Microbiol. 2020 Jan 14;9:461. doi: 10.3389/fcimb.2019.00461. eCollection 2019.
infects millions of individuals worldwide. This protozoan is food and water-borne transmitted but blood transfusion and organ transplantation constitute alternative forms for transmission. However, the influence of IgG anti- antibodies in molecular analysis carried out in peripheral blood still remain unclear. This study aimed to investigate the serum IgG anti- antibody concentrations correlate Nested PCR results in blood donors. 750 blood donors were enrolled. IgM and IgG anti- antibodies were assessed by ELISA (DiaSorin, Italy). Nested PCR was performed with primers JW62/JW63 (288 bp) and B22/B23 (115 bp) of the gene. The mean values of IgG concentration were compared for PCR positive and PCR Negative blood donors using the -test or Mann-Whitney according to the normal distribution (-value ≤ 0.05). 361 (48.1%) blood donors presented positive serology as follow: IgM/IgG: 5 (0.6%); IgM/IgG: 21 (2.8%); IgM/IgG: 335 (44.7%) and 389 (51.9%), negative serology. From 353 blood donors with positive serology tested, the Nested PCR was positive in 38 (10.8%) and negative in 315 (89.2%). There were no differences statistically significant between the mean values of serum IgG anti- antibody concentrations and the Nested PCR results. In conclusion, our data show that variations in the serum IgG anti- antibody concentrations do not correlate parasitemia detected by Nested PCR in chronically infected healthy blood donors.
该原生动物感染全球数百万人。这种原生动物通过食物和水传播，但输血和器官移植构成了传播的替代形式。然而，外周血中分子分析中 IgG 抗-抗体的影响仍不清楚。本研究旨在调查献血者血液中巢式 PCR 结果与血清 IgG 抗-抗体浓度的相关性。共纳入 750 名献血者。采用 ELISA（意大利 DiaSorin）检测 IgM 和 IgG 抗-抗体。采用 JW62/JW63（288 bp）和 B22/B23（115 bp）引物进行巢式 PCR。采用 -检验或 Mann-Whitney 检验（-值≤0.05）比较 PCR 阳性和 PCR 阴性献血者 IgG 浓度的平均值。361（48.1%）名献血者血清学呈阳性，如下所示：IgM/IgG：5（0.6%）；IgM/IgG：21（2.8%）；IgM/IgG：335（44.7%）和 389（51.9%），血清学阴性。在 353 名血清学阳性的献血者中，巢式 PCR 阳性 38 例（10.8%），阴性 315 例（89.2%）。血清 IgG 抗-抗体浓度和巢式 PCR 结果之间的平均值无统计学差异。总之，我们的数据表明，慢性感染的健康献血者中，血清 IgG 抗-抗体浓度的变化与巢式 PCR 检测到的寄生虫血症无关。
