Sardarian K, Maghsood A H, Farimani M, Hajilooi M, Saidijam M, Ghane Z Zarei, Mahaki H, Zamani A
Clin Lab. 2019 Jan 1;65(1). doi: 10.7754/Clin.Lab.2018.180425.
The onset of acute toxoplasmosis in pregnant women may pose a risk to their growing fetuses. The timely diagnosis of infection in managing the disease and preventing its harmful consequences on the fetus is very important. Therefore, the study was conducted to identify acute toxoplasmosis in the pregnant women by detecting the specific IgM antibody and Toxoplasma gondii B1 gene.
A total of 653 serum samples of women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for IgG antibodies against Toxoplasma gondii by enzyme-linked immunosorbent as-say (ELISA). The IgG positive specimens were further examined for IgM by ELISA and polymerase chain reaction (PCR) for B1 gene. In the second phase, change in IgG titers was evaluated on 47 IgG positive samples after two weeks.
ELISA data showed that 167 out of 653 and 2 out of 167 samples were positive for IgG (25.6%) and IgM (1.2%), respectively. However, PCR detection showed that 36 cases (21.6%) were positive for the B1 gene. Seven out of 47 IgG positive samples showed an increase in the antibody titer and positive for the B1 gene. The most cases of IgG positives and the B1 gene samples were associated with the third trimester of pregnancy with 49.7% and 14%, respectively, and the most common abundance of the B1 gene was 14.4% in the age group of 26 - 35. The most commonly reported clinical symptoms in the B1 gene-positive women were nausea 15 (41.7%), cough 13 (36.1%), headache 12 (33.3%), and vomiting 11 (30.5%).
Using PCR and the B1 gene in serum samples of pregnant women to detect acute toxoplasmosis is a more appropriate and accurate method than IgM antibody.
孕妇急性弓形虫病的发作可能对其发育中的胎儿构成风险。及时诊断感染对于控制疾病以及预防其对胎儿的有害后果非常重要。因此，本研究旨在通过检测特异性IgM抗体和弓形虫B1基因来识别孕妇中的急性弓形虫病。
对参加哈马丹医科大学法特米耶医院的653名女性的血清样本进行酶联免疫吸附测定（ELISA），检测抗弓形虫IgG抗体。IgG阳性标本进一步通过ELISA检测IgM，并通过聚合酶链反应（PCR）检测B1基因。在第二阶段，对47份IgG阳性样本在两周后评估IgG滴度的变化。
ELISA数据显示，653份样本中有167份IgG阳性（25.6%），167份样本中有2份IgM阳性（1.2%）。然而，PCR检测显示36例（21.6%）B1基因阳性。47份IgG阳性样本中有7份抗体滴度升高且B1基因阳性。IgG阳性和B1基因样本的大多数病例分别与妊娠晚期相关，比例分别为49.7%和14%，B1基因最常见的丰度在26 - 35岁年龄组中为14.4%。B1基因阳性女性中最常报告的临床症状为恶心15例（41.7%）、咳嗽13例（36.1%）、头痛12例（33.3%）和呕吐11例（30.5%）。
在孕妇血清样本中使用PCR和B1基因检测急性弓形虫病比IgM抗体检测是一种更合适、更准确的方法。
