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PI3K/Akt信号通路对胃癌细胞中PRAS40Thr246磷酸化的影响

Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells.

作者信息

Lu Yizhuo, Li Lianghui, Wu Guoyang, Zhuo Huiqin, Liu Guoyan, Cai Jianchun

机构信息

Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China.

Institute of Gastrointestinal Oncology, School of Medcine, Xiamen University, Xiamen, P.R. China.

出版信息

Iran J Public Health. 2019 Dec;48(12):2196-2204.

PMID:31993387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6974862/
Abstract

BACKGROUND

We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells.

METHODS

The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three groups: gastric cancer cell group, LY294002 group and MK-2206 group. Specific tests were conducted accordingly.

RESULTS

Inhibition of PI3K/Akt signaling pathway activation and PRAS40Thr246 phosphorylation could inhibit proliferation and invasion and promote apoptosis of gastric cancer cells, and PRAS40Thr246 phosphorylation could activate PI3K/Akt signaling pathway.

CONCLUSION

The levels of PI3K/Akt signaling pathway related proteins and p-PRAS40Thr246 were significantly increased in gastric cancer cells. p-PRAS40-Thr246 was able to reflect the activation of the PI3K/Akt signaling pathway, reflecting the sensitivity of the PI3K/AKT signaling pathway to inhibitors.

摘要

背景

我们旨在研究PI3K/Akt信号通路对胃癌细胞中PRAS40苏氨酸246位点磷酸化的影响。

方法

本研究于2017年4月至2018年1月在厦门大学附属中山医院（中国厦门）开展。胃癌细胞被分为三组：胃癌细胞组、LY294002组和MK-2206组。并据此进行了特定检测。

结果

抑制PI3K/Akt信号通路激活和PRAS40苏氨酸246位点磷酸化可抑制胃癌细胞的增殖和侵袭并促进其凋亡，且PRAS40苏氨酸246位点磷酸化可激活PI3K/Akt信号通路。

结论

胃癌细胞中PI3K/Akt信号通路相关蛋白及p-PRAS40苏氨酸246位点水平显著升高。p-PRAS40-苏氨酸246能够反映PI3K/Akt信号通路的激活情况，体现PI3K/AKT信号通路对抑制剂的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/633de39ebf0a/IJPH-48-2196-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/62fccda39cc7/IJPH-48-2196-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/55f6cc79e75b/IJPH-48-2196-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/0390334a9e0b/IJPH-48-2196-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/a38d4972d157/IJPH-48-2196-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/e13b8a9a47b0/IJPH-48-2196-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/c37769c94916/IJPH-48-2196-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/633de39ebf0a/IJPH-48-2196-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/62fccda39cc7/IJPH-48-2196-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/55f6cc79e75b/IJPH-48-2196-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/0390334a9e0b/IJPH-48-2196-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/a38d4972d157/IJPH-48-2196-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/e13b8a9a47b0/IJPH-48-2196-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/c37769c94916/IJPH-48-2196-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/871c/6974862/633de39ebf0a/IJPH-48-2196-g007.jpg

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本文引用的文献

1
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FASEB J. 2018 Sep;32(9):4984-4999. doi: 10.1096/fj.201701096RR. Epub 2018 Apr 17.
2
Outcomes of screening gastroscopy in first-degree relatives of patients fulfilling hereditary diffuse gastric cancer criteria.符合遗传性弥漫性胃癌标准的患者一级亲属行筛查性胃镜检查的结果。
Gastrointest Endosc. 2018 Feb;87(2):397-404.e2. doi: 10.1016/j.gie.2017.04.016. Epub 2017 Apr 25.
3
TIFA 以依赖于 RSK 和 PRAS40 的方式促进结直肠癌细胞增殖。
Cancer Sci. 2022 Sep;113(9):3018-3031. doi: 10.1111/cas.15432. Epub 2022 Jun 14.
4
miR-874: An Important Regulator in Human Diseases.微小RNA-874：人类疾病中的重要调节因子。
Front Cell Dev Biol. 2022 Apr 6;10:784968. doi: 10.3389/fcell.2022.784968. eCollection 2022.
5
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J Med Biochem. 2022 Feb 2;41(1):100-107. doi: 10.5937/jomb0-33374.
6
Aberrant Expression of miR-1301 in Human Cancer.miR-1301在人类癌症中的异常表达。
Front Oncol. 2022 Jan 5;11:789626. doi: 10.3389/fonc.2021.789626. eCollection 2021.
7
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9
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5
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6
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7
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8
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Cell Oncol (Dordr). 2016 Feb;39(1):23-33. doi: 10.1007/s13402-015-0247-3. Epub 2015 Oct 12.
9
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10
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