Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, P. R. China.
J Microbiol. 2020 Feb;58(2):142-152. doi: 10.1007/s12275-020-9318-x. Epub 2020 Jan 29.
Pleurotus pulmonarius, a member of the Pleurotaceae family in Basidiomycota, is an edible, economically important mushroom in most Asian countries. In this study, the complete mitochondrial genomes (mtDNA) of three P. pulmonarius strains - two monokaryotic commercial (J1-13 and ZA3) and one wild (X1-15) - were sequenced and analyzed. In ZA3 and X1-15, the mtDNA molecule was found to be a single circle of 68,305 bp and 73,435 bp, respectively. Both strains contain 14 core protein-coding genes and two ribosomal RNA (rRNA) subunit genes. The ZA3 strain has 22 transfer RNA (tRNA) genes and nine introns: eight in cytochrome c oxidase subunit 1 (coxl), and one in the rRNA large subunit (rnl). Monokaryotic J1-13 and ZA3 mtDNAs were found to be similar in their structure. However, the wild strain X1-15 contains 25 tRNA genes and only seven introns in coxl. Open reading frames (ORFs) of ZA3/J1-13 and X1-15 encode LAGLIDADG, ribosomal protein S3, and DNA polymerase II. In addition, mtDNA inheritance in J1-13, ZA3, and X1-15 was also studied. Results showed that the mtDNA inheritance pattern was uniparental and closely related to dikaryotic hyphal location with respect to the parent. Results also show that mtDNA inheritance is influenced by both the parental nuclear genome and mitogenome in the zone of contact between two compatible parents. In summary, this analysis provides valuable information and a basis for further studies to improve our understanding of the inheritance of fungal mtDNA.
双孢蘑菇，属于担子菌门下侧耳科，是亚洲大多数国家中一种可食用且具有经济重要性的蘑菇。在本研究中，我们对三种双孢蘑菇菌株（两个单核商业菌株 J1-13 和 ZA3，以及一个野生菌株 X1-15）的完整线粒体基因组（mtDNA）进行了测序和分析。在 ZA3 和 X1-15 中，mtDNA 分子分别为 68,305 bp 和 73,435 bp 的单环。两个菌株均包含 14 个核心蛋白编码基因和两个核糖体 RNA（rRNA）亚基基因。ZA3 菌株有 22 个转移 RNA（tRNA）基因和九个内含子：八个在细胞色素 c 氧化酶亚基 1（coxl）中，一个在 rRNA 大亚基（rnl）中。单核 J1-13 和 ZA3 的 mtDNA 在结构上相似。然而，野生菌株 X1-15 含有 25 个 tRNA 基因，coxl 中仅含有七个内含子。ZA3/J1-13 和 X1-15 的开放阅读框（ORFs）编码 LAGLIDADG、核糖体蛋白 S3 和 DNA 聚合酶 II。此外，我们还研究了 J1-13、ZA3 和 X1-15 中的 mtDNA 遗传。结果表明，mtDNA 的遗传模式是单亲遗传，与双核菌丝相对于亲本的位置密切相关。结果还表明，mtDNA 的遗传受双亲核基因组和在两个相容亲本接触区的线粒体基因组的共同影响。综上所述，本分析为进一步研究提供了有价值的信息和基础，以提高我们对真菌 mtDNA 遗传的理解。
