Department of Geology, Payame Noor University, Tehran, Iran.
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Recent Pat Biotechnol. 2020;14(2):121-133. doi: 10.2174/1872208314666200128103513.
Genetically engineered microorganisms (GEMs) can be used for bioremediation of the biological pollutants into nonhazardous or less-hazardous substances, at lower cost. Polycyclic aromatic hydrocarbons (PAHs) are one of these contaminants that associated with a risk of human cancer development. Genetically engineered E. coli that encoded catechol 2,3- dioxygenase (C230) was created and investigated its ability to biodecomposition of phenanthrene and pyrene in spiked soil using high-performance liquid chromatography (HPLC) measurement. We revised patents documents relating to the use of GEMs for bioremediation. This approach have already been done in others studies although using other genes codifying for same catechol degradation approach.
In this study, we investigated biodecomposition of phenanthrene and pyrene by a genetically engineered Escherichia coli.
Briefly, following the cloning of C230 gene (nahH) into pUC18 vector and transformation into E. coli Top10F, the complementary tests, including catalase, oxidase and PCR were used as on isolated bacteria from spiked soil.
The results of HPLC measurement showed that in spiked soil containing engineered E. coli, biodegradation of phenanthrene and pyrene comparing to autoclaved soil that inoculated by wild type of E. coli and normal soil group with natural microbial flora, were statistically significant (p<0.05). Moreover, catalase test was positive while the oxidase tests were negative.
These findings indicated that genetically manipulated E. coli can provide an effective clean-up process on PAH compounds and it is useful for bioremediation of environmental pollution with petrochemical products.
基因工程微生物（GEMs）可用于将生物污染物生物修复为非危险或低危害物质，成本更低。多环芳烃（PAHs）是这些污染物之一，与人类癌症发展的风险有关。构建了编码儿茶酚 2,3-双加氧酶（C230）的基因工程大肠杆菌，并使用高效液相色谱（HPLC）测量研究了其在添加土壤中生物降解菲和芘的能力。我们修订了与使用 GEMs 进行生物修复有关的专利文献。尽管使用其他基因编码相同的儿茶酚降解方法，但这种方法已经在其他研究中进行过。
本研究通过基因工程大肠杆菌研究菲和芘的生物降解。
简要地，在将 C230 基因（nahH）克隆到 pUC18 载体并转化到 E. coli Top10F 中之后，使用互补测试，包括过氧化氢酶、氧化酶和 PCR，作为从添加土壤中分离的细菌进行测试。
HPLC 测量结果表明，在含有工程大肠杆菌的添加土壤中，与高温高压灭菌土壤（接种野生型大肠杆菌）和含自然微生物菌群的正常土壤组相比，菲和芘的生物降解具有统计学意义（p<0.05）。此外，过氧化氢酶测试为阳性，而氧化酶测试为阴性。
这些发现表明，基因改造的大肠杆菌可以为 PAH 化合物提供有效的清洁过程，并且对于石化产品环境污染的生物修复很有用。
