Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy.
Department of Medical, Oral and Biotechnological Sciences, "G.d'Annunzio" University of Chieti-Pescara, Italy.
Curr Pharm Des. 2020;26(3):372-375. doi: 10.2174/1381612826666200129091610.
Molecular changes associated with the initiation of the epithelial to mesenchymal transition (EMT) program involve alterations of large proteome-based networks. The role of protein products mapping to non-coding genomic regions is still unexplored.
The goal of this study was the identification of an alternative protein signature in breast cancer cellular models with a distinct expression of EMT markers.
We profiled MCF-7 and MDA-MB-231 cells using liquid-chromatography mass/spectrometry (LCMS/ MS) and interrogated the OpenProt database to identify novel predicted isoforms and novel predicted proteins from alternative open reading frames (AltProts).
Our analysis revealed an AltProt and isoform protein signature capable of classifying the two breast cancer cell lines. Among the most highly expressed alternative proteins, we observed proteins potentially associated with inflammation, metabolism and EMT.
Here, we present an AltProts signature associated with EMT. Further studies will be needed to define their role in cancer progression.
与上皮间质转化 (EMT) 程序启动相关的分子变化涉及对大型蛋白质组为基础的网络的改变。映射到非编码基因组区域的蛋白质产物的作用仍未被探索。
本研究的目的是在具有明显 EMT 标志物表达的乳腺癌细胞模型中鉴定另一种蛋白质特征。
我们使用液相色谱-质谱/质谱 (LCMS/MS) 对 MCF-7 和 MDA-MB-231 细胞进行了分析，并查询了 OpenProt 数据库，以从替代开放阅读框 (AltProts) 中鉴定新的预测同工型和新的预测蛋白质。
我们的分析揭示了一种能够对两种乳腺癌细胞系进行分类的 AltProt 和同工型蛋白质特征。在表达最高的替代蛋白中，我们观察到了可能与炎症、代谢和 EMT 相关的蛋白。
在这里，我们提出了一个与 EMT 相关的 AltProts 特征。需要进一步的研究来确定它们在癌症进展中的作用。
