Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, Colorado 80523, United States.
Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, Colorado 80523, United States.
J Proteome Res. 2020 Mar 6;19(3):1037-1051. doi: 10.1021/acs.jproteome.9b00561. Epub 2020 Feb 25.
Common wheat ( L.) is a global staple crop, and insect pests can impact grain yield. The wheat stem sawfly (, WSS) is a major wheat pest, and while partial resistance has been deployed by breeding for a solid-stem trait, this trait is affected by environment. Here, a proteomics and metabolomics study was performed on four wheat cultivars to characterize a molecular response to WSS infestation. The cultivars Hatcher (hollow-stem partially tolerant), Conan (semisolid-stem-resistant), and Denali and Reeder (hollow-stem-susceptible) were infested with WSS, and changes in stem proteins and metabolites were characterized using liquid chromatography-mass spectrometry. The proteome was characterized as 1830 proteins that included five major biological processes, including metabolic processes and response to stimuli, and the metabolome (1823 metabolites) spanned eight chemical superclasses, including alkaloids, benzenoids, and lipids. All four varieties had a molecular response to WSS following infestation. Hatcher had the most distinct changes, whereby 62 proteins and 29 metabolites varied in metabolic pathways involving enzymatic detoxification, proteinase inhibition, and antiherbivory compound production via benzoxazinoids, neolignans, and phenolics. Taken together, these data demonstrate variation in the wheat stem molecular response to WSS infestation and support breeding for molecular resistance in hollow-stem cultivars.
普通小麦（ L.）是一种全球性的主要粮食作物，而虫害会影响粮食产量。麦茎麦蛾（，WSS）是一种主要的小麦害虫，虽然通过培育实心茎特性进行了部分抗性部署，但该特性受环境影响。在这里，对四个小麦品种进行了蛋白质组学和代谢组学研究，以表征对 WSS 侵袭的分子反应。对感染 WSS 的 Hatcher（空心茎部分耐受）、Conan（半实心茎抗性）、Denali 和 Reeder（空心茎易感性）四个品种的茎蛋白和代谢物进行了特征描述，使用液相色谱-质谱法。蛋白质组学被描述为 1830 种蛋白质，包括五个主要的生物过程，包括代谢过程和对刺激的反应，而代谢组学（1823 种代谢物）涵盖了包括生物碱、苯并呋喃和脂质在内的八个化学超类。所有四个品种在感染 WSS 后都有分子反应。Hatcher 的变化最为明显，其中 62 种蛋白质和 29 种代谢物在涉及酶解毒、蛋白酶抑制和通过苯并恶嗪类、新木脂素和酚类化合物产生抗草食化合物的代谢途径中发生了变化。总之，这些数据表明，小麦茎对 WSS 侵袭的分子反应存在差异，并支持在空心茎品种中进行分子抗性的培育。
