School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, People's Republic of China.
Biomed Mater. 2020 Jun 12;15(4):045013. doi: 10.1088/1748-605X/ab712e.
Stem cell-based tissue engineering shows enormous potential for regenerative medicine. Three-dimensional (3D) stem cell culture is the most basic aspect of tissue engineering. However, achievement of a perfect scaffold for highly efficient 3D cell culture is currently still limited. Herein, a new hyaluronic acid hydrogel dual-enzymatically crosslinked by horseradish peroxidase and choline oxidase is developed as a 3D stem cell culture system. This hydrogel possesses superior stability over two months, controllable biodegradability with hyaluronidases, a high swelling ratio exceeding 6000%, and excellent cytocompatibility in vitro and biocompatibility in vivo. More importantly, a long-time and highly cellular activity 3D culture of bone marrow-derived mesenchymal stem cells was achieved in vitro over 20 days. All these encouraging results highlight the great potential of this new hydrogel for 3D culture and tissue engineering.
基于干细胞的组织工程在再生医学中显示出巨大的潜力。三维（3D）干细胞培养是组织工程最基本的方面。然而，目前仍然难以获得高效 3D 细胞培养的完美支架。在此，开发了一种新型的辣根过氧化物酶和胆碱氧化酶双酶交联透明质酸水凝胶作为 3D 干细胞培养系统。该水凝胶在两个月以上具有优异的稳定性、透明质酸酶可控的生物降解性、超过 6000%的高溶胀比以及体外的优良细胞相容性和体内的生物相容性。更重要的是，骨髓间充质干细胞在体外可以长达 20 天进行长时间的高细胞活性 3D 培养。所有这些令人鼓舞的结果突出表明这种新型水凝胶在 3D 培养和组织工程方面具有巨大的潜力。
