Skiada Vasiliki, Avramidou Marianna, Bonfante Paola, Genre Andrea, Papadopoulou Kalliope K
Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Larissa, 41500, Greece.
Department of Life Sciences and Systems Biology, University of Torino, Torino, 10125, Italy.
New Phytol. 2020 Jun;226(5):1429-1444. doi: 10.1111/nph.16457. Epub 2020 Mar 13.
Legumes interact with a wide range of microbes in their root systems, ranging from beneficial symbionts to pathogens. Symbiotic rhizobia and arbuscular mycorrhizal glomeromycetes trigger a so-called common symbiotic signalling pathway (CSSP), including the induction of nuclear calcium spiking in the root epidermis. By combining gene expression analysis, mutant phenotypic screening and analysis of nuclear calcium elevations, we demonstrate that recognition of an endophytic Fusarium solani strain K (FsK) in model legumes is initiated via perception of chitooligosaccharidic molecules and is, at least partially, CSSP-dependent. FsK induced the expression of Lysin-motif receptors for chitin-based molecules, CSSP members and CSSP-dependent genes in Lotus japonicus. In LysM and CSSP mutant/RNAi lines, root penetration and fungal intraradical progression was either stimulated or limited, whereas FsK exudates triggered CSSP-dependent nuclear calcium spiking, in epidermal cells of Medicago truncatula root organ cultures. Our results corroborate CSSP being involved in the perception of signals from other microbes beyond the restricted group of symbiotic interactions sensu stricto.
豆科植物的根系会与多种微生物相互作用，这些微生物涵盖了从有益共生体到病原体的广泛范围。共生根瘤菌和丛枝菌根球囊霉会触发一种所谓的共同共生信号通路（CSSP），包括在根表皮中诱导核钙峰。通过结合基因表达分析、突变体表型筛选以及核钙升高分析，我们证明在模式豆科植物中对内生茄病镰刀菌菌株K（FsK）的识别是通过对壳寡糖分子的感知启动的，并且至少部分依赖于CSSP。FsK诱导了百脉根中基于几丁质分子的溶素基序受体、CSSP成员和CSSP依赖性基因的表达。在LysM和CSSP突变体/RNAi系中，根的穿透和真菌在根内的进展要么受到刺激，要么受到限制，而FsK分泌物在蒺藜苜蓿根器官培养物的表皮细胞中触发了依赖于CSSP的核钙峰。我们的结果证实CSSP参与了对来自严格意义上的共生相互作用受限群体之外的其他微生物信号的感知。
