Kumawat Vivek S, Kaur Ginpreet
Department of Pharmacology, Shobhaben Pratapbhai Patel School of Pharmacy & Technology Management, SVKM's NMIMS, Mumbai, India.
J Food Biochem. 2020 Apr;44(4):e13156. doi: 10.1111/jfbc.13156. Epub 2020 Jan 29.
Beta-caryophyllene (BCP) is a flavoring agent, whereas l-arginine (LA) is used as a food supplement. They possess insulinotropic and β cell regeneration activities, respectively. We assessed the antidiabetic potential of BCP, LA, and its combination in RIN-5F cell lines and diabetic rats. Ex vivo studies were carried out for glucose uptake and absorption of the combination of BCP with LA. The results indicated that the combination of BCP with LA showed a significant decrease in glucose absorption and an increase in its uptake in tissues and also an increase in insulin secretion in RIN-5F cells. The combination treatment of BCP with LA showed a significant reduction in glucose, lipid levels, and oxidative stress in pancreatic tissue when compared with the diabetic group. Furthermore, the combination of BCP with LA normalized glucose tolerance and pancreatic cell damage in diabetic rats. In conclusion, the combinational treatment showed significant potentials in the treatment of type 2 diabetes mellitus. PRACTICAL APPLICATIONS: Type 2 diabetes mellitus is the most prevalent chronic metabolic disorder affecting a large population. Beta-caryophyllene is a CB receptor agonist shown to have insulinotropic activity. l-Arginine is a food supplement that possesses beta-cell regeneration property. The combination of BCP with LA could work as a potential therapeutic intervention, considering the individual pharmacological activities of each. We evaluated the antidiabetic activity of the combination of BCP with LA in diabetic rats using ex vivo and in vitro experimentations. Results from the study revealed that the combination of BCP with LA showed a significant (p < .001) reduction in glucose and lipid levels as compared to individual treatment. In vitro study also supports the diabetic potential of the combination of BCP with LA in the glucose-induced insulin secretion in RIN-5F cell lines. The study indicates a therapeutic approach to treat T2DM by BCP and LA combination as food and dietary supplement.
β-石竹烯（BCP）是一种调味剂，而L-精氨酸（LA）用作食品补充剂。它们分别具有促胰岛素分泌和β细胞再生活性。我们评估了BCP、LA及其组合在RIN-5F细胞系和糖尿病大鼠中的抗糖尿病潜力。对BCP与LA组合进行了葡萄糖摄取和吸收的体外研究。结果表明，BCP与LA的组合使葡萄糖吸收显著降低，组织对葡萄糖的摄取增加，并且RIN-5F细胞中的胰岛素分泌也增加。与糖尿病组相比，BCP与LA的联合治疗使胰腺组织中的葡萄糖、脂质水平和氧化应激显著降低。此外，BCP与LA的组合使糖尿病大鼠的糖耐量和胰腺细胞损伤恢复正常。总之，联合治疗在2型糖尿病的治疗中显示出显著潜力。实际应用：2型糖尿病是影响大量人群的最普遍的慢性代谢紊乱疾病。β-石竹烯是一种CB受体激动剂，具有促胰岛素分泌活性。L-精氨酸是一种具有β细胞再生特性的食品补充剂。考虑到各自的药理活性，BCP与LA的组合可能作为一种潜在的治疗干预措施。我们通过体外和体内实验评估了BCP与LA组合在糖尿病大鼠中的抗糖尿病活性。研究结果表明，与单独治疗相比，BCP与LA的组合使葡萄糖和脂质水平显著降低（p <.001）。体外研究也支持BCP与LA组合在RIN-5F细胞系中葡萄糖诱导的胰岛素分泌方面的抗糖尿病潜力。该研究表明通过BCP和LA组合作为食品和膳食补充剂来治疗2型糖尿病的一种治疗方法。
