YoungLai E V, Borgmann A
Department of Obstetrics and Gynecology, McMaster University Medical Centre, Hamilton, Ontario, Canada.
J Steroid Biochem. 1988 Dec;31(6):983-6. doi: 10.1016/0022-4731(88)90342-1.
The presence of 3 beta-hydroxysteroid dehydrogenase in the maturing rabbit ovary was demonstrated biochemically and histochemically. Enzyme activity was negligible to absent in ovaries from rabbits less than 44 days old. The greatest activity was located in the microsomal fraction of ovaries from mature rabbits. The enzyme characteristics were: Vmax = 33.1 +/- 9.6 nmol/min/mg protein and Km = 2.16 +/- 0.28 microM. Ovaries from pregnant hyperglycemic rabbits had enzyme which showed a Vmax of 51.4 +/- 8.2 nmol/min/mg protein and Km = 2.41 +/- 0.31 microM. These results indicate that rabbit ovarian tissue becomes steroidogenically active at a time when gonadotropin levels are elevated.
通过生物化学和组织化学方法证实了成熟兔卵巢中存在3β-羟基类固醇脱氢酶。在小于44日龄的兔卵巢中，酶活性可忽略不计或不存在。最大活性位于成熟兔卵巢的微粒体部分。酶的特性为：Vmax = 33.1 +/- 9.6 nmol/分钟/毫克蛋白质，Km = 2.16 +/- 0.28微摩尔。妊娠高血糖兔的卵巢中酶的Vmax为51.4 +/- 8.2 nmol/分钟/毫克蛋白质，Km = 2.41 +/- 0.31微摩尔。这些结果表明，在促性腺激素水平升高时，兔卵巢组织开始具有类固醇生成活性。
