Donaubauer Anna-Jasmina, Becker Ina, Rühle Paul F, Fietkau Rainer, Gaipl Udo S, Frey Benjamin
Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Methods Enzymol. 2020;632:389-415. doi: 10.1016/bs.mie.2019.03.003. Epub 2019 Apr 4.
Supplementation of standard cancer therapies (radiotherapy, chemotherapy, surgery) with immunotherapies has revolutionized cancer treatment. In order to include recent improvements of multimodal therapies into clinical routine, knowledge about the immune status, the immune dynamics and the detailed composition and activation of patient's immune system is required. The here presented single-tube multicolor flow cytometry assay allows the discrimination of 20 clinically relevant immune cell subsets and their activation status in peripheral whole blood. It includes 15 different antibodies and can be established on a common 3 laser and 10 color flow-cytometer. Furthermore, this assay is easy to set-up and to perform as well as fast with only 40min of sample preparation time. Moreover, only 100μL of whole blood are sufficient for this precise determination of the individual immune status. It is already applied in translational programs of clinical studies and trials and can further be adapted for future ones.
用免疫疗法补充标准癌症治疗方法（放疗、化疗、手术）彻底改变了癌症治疗方式。为了将多模式疗法的最新进展纳入临床常规，需要了解患者免疫系统的免疫状态、免疫动态以及详细组成和激活情况。本文介绍的单管多色流式细胞术检测方法能够区分外周全血中20种临床相关免疫细胞亚群及其激活状态。它包含15种不同抗体，可在常见的三激光十色流式细胞仪上进行检测。此外，该检测方法易于设置和操作，且速度快，样本制备时间仅需40分钟。而且，仅100μL全血就足以精确测定个体免疫状态。它已应用于临床研究和试验的转化项目中，并且可以进一步适用于未来的项目。
