de Oliveira C A, Mantovani B
Department of Biochemistry, Ribeirão Preto Medical School, University of São Paulo, Brazil.
Life Sci. 1988;43(22):1825-30. doi: 10.1016/0024-3205(88)90282-2.
We have found that latrunculin A, a Red Sea sponge toxin, is a potent inhibitor of immunological phagocytosis by mouse peritoneal macrophages, but does not block the binding (recognition) of the immune complexes (erythrocytes sensitized with IgG antibodies) to the cells. The inhibition begins to be appreciable around 12 nM latrunculin A, and is complete with a toxin concentration of 60 nM. This inhibitory effect does not interfere with the cell viability, and can be reversed when the macrophages are incubated in fresh medium. Since latrunculin A is a disrupting agent of microfilament organization, these results strengthen the evidence for the active participation of microfilaments in the mechanism of phagocytosis and at the same time provide a new tool for the investigation of phagocytosis at the molecular level.
我们发现，红海海绵毒素拉春库林A是小鼠腹腔巨噬细胞免疫吞噬作用的强效抑制剂，但不会阻断免疫复合物（用IgG抗体致敏的红细胞）与细胞的结合（识别）。在拉春库林A浓度约为12 nM时，抑制作用开始明显，毒素浓度为60 nM时抑制作用完全。这种抑制作用不影响细胞活力，当巨噬细胞在新鲜培养基中孵育时，抑制作用可逆转。由于拉春库林A是微丝组织的破坏剂，这些结果进一步证明了微丝在吞噬作用机制中的积极参与，同时为在分子水平上研究吞噬作用提供了一种新工具。
