用 Flyception2 对果蝇的复杂社会行为进行大脑活动成像。

Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, CA, USA.

Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, CA, USA.

Nat Commun. 2020 Jan 30;11(1):623. doi: 10.1038/s41467-020-14487-7.

Optical in vivo recordings from freely walking Drosophila are currently possible only for limited behaviors. Here, we expand the range of accessible behaviors with a retroreflective marker-based tracking and ratiometric brain imaging system, permitting brain activity imaging even in copulating male flies. We discover that P1 neurons, active during courtship, are inactive during copulation, whereas GABAergic mAL neurons remain active during copulation, suggesting a countervailing role of mAL in opposing P1 activity during mating.

目前，只能对有限行为的自由行走的果蝇进行体内光学记录。在这里，我们通过基于反射标记的跟踪和比率脑成像系统扩展了可访问行为的范围，即使在交配的雄性果蝇中也允许进行大脑活动成像。我们发现，在求偶过程中活跃的 P1 神经元在交配过程中不活跃，而 GABA 能 mAL 神经元在交配过程中保持活跃，这表明 mAL 在交配过程中对抗 P1 活动中起着抵消作用。