Hata K, Ono E, Yanagawa R
Department of Hygiene and Microbiology, Faculty of Veterinary Medicine, Hokkaido University.
Microbiol Immunol. 1988;32(8):817-32. doi: 10.1111/j.1348-0421.1988.tb01443.x.
Strain Ictero No. I, the first isolate of Leptospira, isolated by Inada and Ido in 1914, was found to be sufficiently qualified to be the type strain of Leptospira interrogans rather than strain RGA. In an agglutinin absorption test, anti-Ictero No. I serum was not absorbed completely with strain RGA, and 25% of the homologous titer remained unabsorbed, while anti-RGA serum was completely absorbed with strain Ictero No. I. Thus, strain Ictero No. I was not serologically identical with strain RGA, and the two strains were considered to be different serovars. A protein band with a molecular weight of approximately 33,000 daltons was detected in strain Ictero No. I but not in strain RGA by SDS-PAGE. By Western blotting, this protein band was detectable with anti-Ictero No. I serum but not with anti-RGA serum. The presence of the 33K protein in strain Ictero No. I, but not in strain RGA, was confirmed by radioimmunoprecipitation using [125I]-labeled antigens, indicating that the protein antigen was surface-exposed. Only 8 of the 89 enzymes activities were different between strains Ictero No. I and RGA (line Sapporo). From the above results, we propose that strain Ictero No. I should be designated as the type strain of L. interrogans instead of strain RGA.
黄疸出血群菌株I号（Ictero No. I）是1914年稻田和伊东分离出的第一株钩端螺旋体，被认为完全有资格作为问号钩端螺旋体（Leptospira interrogans）的模式菌株，而非RGA菌株。在凝集素吸收试验中，抗黄疸出血群菌株I号血清不能被RGA菌株完全吸收，25%的同源效价仍未被吸收，而抗RGA血清则能被黄疸出血群菌株I号完全吸收。因此，黄疸出血群菌株I号与RGA菌株血清学上不相同，这两个菌株被认为是不同的血清型。通过SDS-PAGE在黄疸出血群菌株I号中检测到一条分子量约为33,000道尔顿的蛋白带，而在RGA菌株中未检测到。通过蛋白质印迹法，这条蛋白带能用抗黄疸出血群菌株I号血清检测到，而不能用抗RGA血清检测到。使用[125I]标记抗原的放射免疫沉淀法证实黄疸出血群菌株I号中存在33K蛋白，而RGA菌株中不存在，表明该蛋白抗原位于表面。黄疸出血群菌株I号和RGA菌株（札幌株系）之间只有89种酶活性中的8种不同。基于上述结果，我们提议黄疸出血群菌株I号应被指定为问号钩端螺旋体的模式菌株，而非RGA菌株。
