Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168, Messina, Italy.
Chromaleont s.r.l., via Ludovisi 45, 00187, Rome, Italy.
Anal Bioanal Chem. 2020 Apr;412(10):2327-2337. doi: 10.1007/s00216-020-02424-y. Epub 2020 Jan 30.
The qualitative and quantitative profiling of fatty acids in human blood is a useful tool in disease prevention and health care, two concepts that are intimately related. In fact, fatty acid (FA) analysis can provide in-depth information on a specific metabolic state of individuals. The goal of the present research consisted of the development of a rapid and miniaturized analytical strategy for the complete characterization of the fatty acid profile in human blood. Sample collection was carried out by using the dried blood spot approach, while fatty acid derivatization to methyl esters was performed directly by using sodium methoxide and boron trifluoride. The following figures of merit were defined: intra- and inter-day repeatability, linearity range, limits of detection, and quantification. Additionally, the accuracy of the developed method was evaluated in the analysis of a certified reference human plasma sample. Apart from blood, the analytical procedure was also applied to samples of human serum and plasma. During the final stage of the research, the developed analytical method was performed in a fully automated manner. Graphical abstract.
人血液中脂肪酸的定性和定量分析是疾病预防和保健的有用工具，这两个概念密切相关。事实上，脂肪酸（FA）分析可以提供个体特定代谢状态的深入信息。本研究的目的是开发一种快速和微型化的分析策略，以全面描述人血液中的脂肪酸谱。通过使用干血斑法进行样本采集，而直接使用甲醇钠和三氟化硼将脂肪酸衍生为甲酯。定义了以下评价指标：日内和日间重复性、线性范围、检测限和定量限。此外，还评估了该方法在分析认证参考人类血浆样本中的准确性。除了血液外，该分析程序还应用于人类血清和血浆样本。在研究的最后阶段，以全自动方式执行所开发的分析方法。
