Department of Chemistry and Biochemistry, University of Texas Arlington, Arlington, TX, USA.
VUV Analytics, Inc., Cedar Park, TX, USA.
Anal Chim Acta. 2019 Apr 11;1053:169-177. doi: 10.1016/j.aca.2018.12.007. Epub 2018 Dec 15.
Qualitative and quantitative determination of fatty acids in plasma is of extreme importance as these are indicators of metabolic diseases. In this work, a sensitive and rugged method for detecting and quantifying fatty acids (as fatty acid methyl ester derivatives, FAMEs) in blood plasma was developed. The use of large-volume injection (LVI) gas chromatography-vacuum ultraviolet spectroscopy (GC-VUV) for analysis of fatty acids in blood plasma allowed the injection of higher sample volumes to accommodate sufficient analyte on-column for necessary detection ranges with a run time of 45 min. Calibration curves exhibited consistent linearity and reproducibility and were used along with internal standards for the quantification of 11 saturated and 21 unsaturated fatty acids. Intra-day and inter-day (n = 6) CVs had an average of 5 and 6%, respectively, and recoveries an average of 105%. The concentrations of EPA, DHA, and AA, as well as the omega-3 index and omega-6/omega-3 ratio, were calculated and compared with clinically actionable measurement ranges. Due to the use of LVI, the more volatile analyte (C8:0) was lost and therefore impossible to quantify. The volatility cutoff was determined to be the C10:0 analyte with a molecular weight of 186.295 g/mol.
定性和定量测定血浆中的脂肪酸极为重要，因为它们是代谢疾病的指标。在这项工作中，开发了一种用于检测和定量分析血液中脂肪酸（作为脂肪酸甲酯衍生物，FAMEs）的灵敏而坚固的方法。使用大体积进样（LVI）气相色谱-真空紫外光谱（GC-VUV）分析血液中的脂肪酸，允许注入更高的样品体积，以在 45 分钟的运行时间内为必要的检测范围提供足够的柱上分析物。校准曲线表现出一致的线性和重现性，并与内标一起用于定量分析 11 种饱和脂肪酸和 21 种不饱和脂肪酸。日内和日间（n=6）的 CV 平均值分别为 5%和 6%，回收率平均值为 105%。计算了 EPA、DHA 和 AA 的浓度，以及 omega-3 指数和 omega-6/omega-3 比值，并与临床可操作的测量范围进行了比较。由于使用了 LVI，更易挥发的分析物（C8:0）被损失，因此无法定量。挥发性截止值被确定为分子量为 186.295 g/mol 的 C10:0 分析物。
