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甘露聚糖修饰的菊粉微球增强口蹄疫多表位亚单位疫苗的免疫效力。

Enhanced Efficacy of Immunization with a Foot-and-Mouth Disease Multi-Epitope Subunit Vaccine Using Mannan-Decorated Inulin Microparticles.

机构信息

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.

Institute of Green-Bio Science and Technology, Seoul National University, 1447 Pyeongchang-daero, Daehwa-myeon, Pyeongchang-Gun, Gangwon-do, 25354, Republic of Korea.

出版信息

Tissue Eng Regen Med. 2020 Feb;17(1):33-44. doi: 10.1007/s13770-019-00228-5. Epub 2019 Dec 9.

Abstract

BACKGROUND

Despite the many advantages of recombinant subunit vaccines, they have critical weaknesses that include a low efficacy for promoting cellular and humoral immune responses against antigens because of their poor immunogenicity, and a rapidly cleared properties as a result of proteolytic enzymes in the body. To circumvent these problems, we developed mannan-decorated inulin acetate microparticles (M-IA MPs) that functioned as carriers and adjuvants for immunization with the recombinant foot-and-mouth disease multi-epitope subunit vaccine (M5BT).

METHODS

The M5BT-loaded M-IA MPs were obtained by a double-emulsion solvent-evaporation method. Their properties including morphology, size and release ability were determined by field emission scanning electron microscope, dynamic light-scattering spectrophotometer and spectrophotometer. To assess the immunization efficacy of the MPs, mice were immunized with MPs and their sera were analyzed by ELISA.

RESULTS

The M-IA MPs obtained by a double-emulsion solvent-evaporation method were spherical and approximately 2-3 µm, and M5BT was encapsulated in the M-IA MPs. The M5BT-loaded M-IA MPs showed higher antigen-specific IgG, IgG1, IgG2a and anti-FMDV antibodies than the M5BT-loaded IA MPs and the Freund's adjuvant as a control.

CONCLUSION

The M-IA MPs showed a powerful and multifunctional polymeric system that combined two toll-like receptor agonists compared to the conventional adjuvant.

摘要

背景

尽管重组亚单位疫苗有许多优点,但它们也存在一些关键的弱点,包括由于其免疫原性差,对促进细胞和体液免疫应答抗原的效力较低,以及由于体内的蛋白水解酶而导致的迅速清除特性。为了规避这些问题,我们开发了甘露聚糖修饰的醋酸菊粉微球(M-IA MPs),它们作为免疫接种口蹄疫多表位重组亚单位疫苗(M5BT)的载体和佐剂。

方法

采用双乳液溶剂蒸发法获得负载 M5BT 的 M-IA MPs。通过场发射扫描电子显微镜、动态光散射分光光度计和分光光度计来确定其形态、大小和释放能力。为了评估 MPs 的免疫效果,用 MPs 免疫小鼠,并通过 ELISA 分析其血清。

结果

采用双乳液溶剂蒸发法得到的 M-IA MPs 呈球形,大小约为 2-3 µm,并且 M5BT 被包裹在 M-IA MPs 中。与负载 M5BT 的 IA MPs 和作为对照的弗氏佐剂相比,负载 M5BT 的 M-IA MPs 表现出更高的抗原特异性 IgG、IgG1、IgG2a 和抗口蹄疫病毒抗体。

结论

与传统佐剂相比,M-IA MPs 显示出一种强大的多功能聚合物系统,结合了两种 Toll 样受体激动剂。

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