Division of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
Institute of Agricultural Science and Technology, Kyungpook National University, Daegu, Republic of Korea.
Pest Manag Sci. 2020 Jul;76(7):2347-2354. doi: 10.1002/ps.5770. Epub 2020 Feb 12.
Methyl benzoate (MB) is a small, hydrophobic organic compound isolated from the freshwater fern Salvinia molesta (Salviniales: Salviniaceae). It is used as a fragrance and flavor enhancer owing to its pleasant smell. It has also demonstrated potential as a green pesticide for various groups of insects. However, its effects on mites are yet to be studied.
Here, we assessed the acaricidal and repellent effects of MB against the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), under laboratory and greenhouse conditions. MB demonstrated concentration-dependent contact toxicity against eggs and adults of the mite. A leaf-dipping assay using 1% MB prevented the hatching of 92.7% of eggs and killed 100% of adults within 48 h of treatment. Concentration-mortality statistics were subjected to probit analysis, and the median lethal concentration (LC ) values for eggs and adults were 0.25% and 0.5%, respectively. Treatment with 1% MB showed the highest mortality (100%), with a median lethal time (LT ) estimated of 8.1 h. The efficacy of MB against adults of T. urticae on tomato plants under greenhouse conditions was 97.5% within 96 h post-treatment. Further, MB showed significant repellent activity against adult females of T. urticae, although this declined with time. Spraying with 1% MB (three times per plant) was not phytotoxic to bean, cucumber, pepper, or tomato plants.
MB is highly acaricidal and repellent, but not phytotoxic, and is a promising green pesticide.
苯甲酸甲酯（MB）是一种从淡水蕨类植物满江红（Salviniales：满江红科）中分离出来的小而疏水的有机化合物。由于其气味宜人，它被用作香料和风味增强剂。它还表现出作为各种昆虫群体的绿色杀虫剂的潜力。然而，它对螨虫的影响尚未得到研究。
在这里，我们评估了 MB 对二斑叶螨（Tetranychus urticae Koch）（蜱螨目：叶螨科）的杀螨和驱避作用，在实验室和温室条件下进行。MB 对螨的卵和成虫表现出浓度依赖性接触毒性。使用 1% MB 的叶片浸渍测定法阻止了 92.7%的卵孵化，并在处理后 48 小时内杀死了 100%的成虫。浓度死亡率统计数据进行了概率分析，卵和成虫的半数致死浓度（LC ）值分别为 0.25%和 0.5%。用 1% MB 处理显示出最高的死亡率（100%），估计的半数致死时间（LT ）为 8.1 小时。在温室条件下，MB 对番茄植株上的 T. urticae 成虫的防治效果在处理后 96 小时内达到 97.5%。此外，MB 对 T. urticae 雌成虫表现出显著的驱避活性，尽管随着时间的推移驱避活性下降。向植物上喷洒 1% MB（每次每株植物喷洒三次）对豆类、黄瓜、胡椒或番茄植物没有植物毒性。
MB 具有高度的杀螨和驱避作用，但没有植物毒性，是一种很有前途的绿色杀虫剂。
