Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
Research laboratory for Epidemiology and immunogenetics of viral infections (LR14SP02), Sahloul University Hospital, University of Sousse, Sousse, Tunisia.
J Med Microbiol. 2020 Feb;69(2):280-289. doi: 10.1099/jmm.0.001156. Epub 2020 Jan 31.
Group A Rotavirus (RVA) is known to be a major cause of acute gastroenteritis (AGE) in children but its role as a potential pathogen in immunocompetent adults is probably underestimated. To compare RVA infections in patients from different age groups. Fecal samples were collected from patients aged from birth to 65 years, hospitalized or consulting for AGE between 2015 and 2017. All samples were screened by RT-PCR for the detection of VP6 gene specific of RVA. RVA-positive samples were VP7 and VP4 genotyped using multiplex semi-nested RT-PCR. Full-length VP7 gene of G9-positive strains were sequenced and submitted for phylogenetic analysis. Of 1371 stool specimens collected from children (<5 years; =454), older children (5 to <15 years; =316) and adults (15-65 years; =601), 165 (12.0 %) were RVA-positive. RVA detection rates were significantly higher in children and adults than in older children (15.8 % and 12.1 Vs 6.3 %, respectively; <0.001). While RVA infections were mostly detected during the coldest months in children, they were observed all year-round in patients aged >5 years. Although G1P[8] remained the most prevalent combination (41.7 %) detected in children, G9P[8] strains widely predominated in adults (58.1 %), followed by G2P[4] (12.9 %). All characterized G9 strains clustered in the modern lineage III. RVA play an important role in AGE not only in children but also in adults. The findings of a wide G9 predominance in patients >5 years highlights the need for continuing surveillance in both pediatric and mature populations.
A 组轮状病毒（RVA）已知是导致儿童急性肠胃炎（AGE）的主要原因，但它作为免疫功能正常成年人的潜在病原体的作用可能被低估了。为了比较不同年龄组患者的 RVA 感染情况。收集了 2015 年至 2017 年间因 AGE 住院或就诊的出生至 65 岁患者的粪便样本。所有样本均通过 RT-PCR 检测 RVA 的 VP6 基因进行筛查。用多重半巢式 RT-PCR 对 RVA 阳性样本进行 VP7 和 VP4 基因分型。对 G9 阳性株的全长 VP7 基因进行测序，并提交进行系统进化分析。从 1371 份来自儿童（<5 岁；=454）、较大儿童（5 至<15 岁；=316）和成年人（15-65 岁；=601）的粪便标本中，165 份（12.0%）为 RVA 阳性。儿童和成人的 RVA 检出率明显高于较大儿童（15.8%和 12.1%比 6.3%；<0.001）。虽然 RVA 感染主要在儿童的最寒冷月份检测到，但在>5 岁的患者中全年都有观察到。虽然 G1P[8]仍然是儿童中最常见的组合（41.7%），但 G9P[8]株在成年人中广泛占主导地位（58.1%），其次是 G2P[4]（12.9%）。所有特征化的 G9 株都聚集在现代谱系 III 中。RVA 在 AGE 中不仅在儿童中而且在成人中都发挥着重要作用。>5 岁患者中广泛存在 G9 优势的发现强调了在儿科和成人人群中继续进行监测的必要性。
