State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, Strait Collaborative Innovation Center of Biomedicine and Pharmaceutics, School of Public Health, Xiamen University, Xiamen, China.
Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing, China.
J Med Virol. 2019 Oct;91(10):1788-1796. doi: 10.1002/jmv.25530. Epub 2019 Jul 12.
Human group A rotavirus (RVA) is the leading cause of acute viral gastroenteritis in children under 5 years old worldwide. The aim of this study was to investigate the genotype distribution of RVA in the Midwest of China. Sentinel-based surveillance of acute diarrhea was conducted at Children's Hospital of Chongqing Medical University from 2011 to 2015. RVA was tested by using enzyme-linked immunosorbent assays. The partial VP4 genes and VP7 genes of rotavirus were amplified and sequenced, and genotyping and phylogenetic analyses were performed. Among the 2236 stool specimens collected from children with acute gastroenteritis, 681 (30.46%) were positive for RVA. The majority of children (89.28%) who tested positive for RVA were children aged ≤2 years. The seasonal peak of RVA was in the winter. As for genotype, four strain combinations, G9P[8], G3P[8], G1P[8], and G2P[4] contributed to 75.62% (515/681) of the RVA-associated diarrhea cases. After a marked increase in G9P[8] (30.77%) in 2013, G9P[8] became the predominant genotype in 2014 and 2015, whilst the prevalence of G1P[8] was decreased to 2.72% in 2015. Unusual G-P combinations (eg, G1P[4], G9P[4], G4P[6], G3P[4], G2P[8]) were also detected sporadically over the study period. Phylogenetic tree analysis results showed that the VP7 sequences of G9 strains were clustered into two main lineages, and 77.34% of them were clustered into lineage VI, with the highest nucleotide similarity to the strain JS12-17(China). VP4 gene sequences of P[8] strains were almost P[8]-lineage 3. Substantial temporal variation in the circulation of various genotypes of rotavirus in Chongqing was observed during 2011-2015, and highlights the need for continuous surveillance of RVA infection for better understanding and control of RVA infection.
人类 A 组轮状病毒（RVA）是全球 5 岁以下儿童急性病毒性胃肠炎的主要病因。本研究旨在调查中国中西部地区 RVA 的基因型分布。2011 年至 2015 年，重庆医科大学儿童医院开展了基于哨点的急性腹泻监测。采用酶联免疫吸附试验检测 RVA。扩增并测序轮状病毒部分 VP4 基因和 VP7 基因，进行基因分型和系统进化分析。在 2236 份急性胃肠炎患儿粪便标本中，681 份（30.46%）为 RVA 阳性。89.28%的 RVA 阳性患儿为≤2 岁儿童。RVA 的季节性高峰在冬季。就基因型而言，四种毒株组合，G9P[8]、G3P[8]、G1P[8]和 G2P[4]占 RVA 相关腹泻病例的 75.62%（515/681）。2013 年 G9P[8]（30.77%）显著增加后，G9P[8]成为 2014 年和 2015 年的主要基因型，而 G1P[8]的流行率在 2015 年降至 2.72%。在整个研究期间，还偶尔检测到不常见的 G-P 组合（如 G1P[4]、G9P[4]、G4P[6]、G3P[4]、G2P[8]）。系统进化树分析结果显示，G9 株的 VP7 序列聚为两个主要分支，其中 77.34%聚为第六分支，与 JS12-17（中国）株的核苷酸相似度最高。P[8]株的 VP4 基因序列几乎均为 P[8]-3 分支。2011-2015 年间，重庆各种轮状病毒基因型的传播存在明显的时间变化，这突出表明需要对 RVA 感染进行持续监测，以便更好地了解和控制 RVA 感染。
