Onoda N
Department of Physiology, School of Medicine, Gunma University, Japan.
Neuroscience. 1988 Sep;26(3):1013-22. doi: 10.1016/0306-4522(88)90116-9.
Degeneration and regeneration of olfactory receptor neurons were studied in adult rabbits by immunohistochemical procedures following unilateral olfactory bulbectomy. Staining patterns of the olfactory receptors of the lesioned side were compared with those of the intact side in the nasal septum at various postoperative periods (12h-6 months) following lesion. Monoclonal antibodies, produced against the rabbit olfactory bulb, were used as histochemical markers. A slight decrease in the number of olfactory receptor neurons occurred at 24 h after lesion. One monoclonal antibody 112D5 stained all receptor neurons including degenerating neurons, but the other 114G12 showed a rapid decrease in immunostaining so that 114G12-positive cells disappeared within 7 days after lesion. 114G12-positive cells reappeared at 4 weeks following lesion. By 3 months, 114G12-positive cells were arranged in a plane at the apical region of the superficial compartment of the receptor cell layer, suggesting a recapitulation of development pattern of the receptor neurons. Thereafter, the number of 114G12-positive cells increased progressively and the staining pattern of the olfactory epithelium was like that of control animals by 6 months. Monoclonal antibody 114G12 is thus the first marker that is not specific to olfactory neurons and can be used to characterize certain embryonic traits during the degeneration and regeneration of the olfactory epithelium in the adult mammal.
采用免疫组织化学方法，在成年兔单侧嗅球切除术后，研究嗅感觉神经元的退变与再生。在损伤后的不同术后时期（12小时至6个月），比较损伤侧与鼻中隔完整侧嗅感受器的染色模式。使用针对兔嗅球产生的单克隆抗体作为组织化学标记物。损伤后24小时，嗅感觉神经元数量略有减少。一种单克隆抗体112D5可标记所有的感受器神经元，包括退变的神经元，但另一种单克隆抗体114G12的免疫染色则迅速减弱，以至于损伤后7天内114G12阳性细胞消失。损伤后4周，114G12阳性细胞重新出现。到3个月时，114G12阳性细胞排列在感受器细胞层浅部的顶端区域，呈平面状，提示感受器神经元发育模式的重现。此后，114G12阳性细胞数量逐渐增加，到6个月时，嗅上皮的染色模式与对照动物相似。因此，单克隆抗体114G12是首个并非嗅神经元特异性的标记物，可用于表征成年哺乳动物嗅上皮退变与再生过程中的某些胚胎特征。
