Onoda N
Department of Physiology, School of Medicine, Gunma University, Japan.
Neuroscience. 1988 Sep;26(3):1003-12. doi: 10.1016/0306-4522(88)90115-7.
Staining patterns for monoclonal antibodies produced against the rabbit olfactory bulb were studied from embryonic day 14 up to 30 days after birth in the rabbit olfactory receptor neurons. One monoclonal antibody, 112D5, stained all of the receptor neurons in the olfactory epithelium and vomeronasal organ during development. The other, 114G12, showed a unique gradual expression in the olfactory receptor neurons. 114G12-Positive cells first appeared in the epithelium of the embryonic day-17 fetus. At embryonic day 25 or 26, 114G12-positive cells were situated in the superficial receptor cell layer. The arrangement in the positive and negative receptor neurons was 'superficial-positive' and 'deep-negative'. Thereafter, a gradual increase in the number of 'superficial-positive' cells was accompanied by a decrease in the 'deep-negative' cells. These changes continued until postnatal day 30. The negativity of staining for monoclonal antibody 114G12 in the deep compartment was retained in the adult rabbit. The supporting cells and basal cells were monoclonal antibody 114G12-negative throughout development. This unique developmental pattern suggests that monoclonal antibody 114G12 is a useful probe for studies on neurogenesis in adult animal. In contrast to the olfactory epithelium, the vomeronasal organ was monoclonal antibody 114G12-negative throughout development. Thus monoclonal antibody 114G12 makes a molecular distinction between the olfactory receptor neurons and vomeronasal system.
在兔嗅觉受体神经元中，研究了从胚胎第14天到出生后30天针对兔嗅球产生的单克隆抗体的染色模式。一种单克隆抗体112D5在发育过程中对嗅觉上皮和犁鼻器中的所有受体神经元进行染色。另一种单克隆抗体114G12在嗅觉受体神经元中呈现独特的逐渐表达模式。114G12阳性细胞最早出现在胚胎第17天胎儿的上皮中。在胚胎第25或26天，114G12阳性细胞位于浅表受体细胞层。阳性和阴性受体神经元的排列方式为“浅表阳性”和“深部阴性”。此后，“浅表阳性”细胞数量逐渐增加，同时“深部阴性”细胞数量减少。这些变化一直持续到出生后第30天。成年兔深部区域对单克隆抗体114G12的染色阴性得以保留。支持细胞和基底细胞在整个发育过程中对单克隆抗体114G12均呈阴性。这种独特的发育模式表明，单克隆抗体114G12是研究成年动物神经发生的有用探针。与嗅觉上皮不同，犁鼻器在整个发育过程中对单克隆抗体114G12均呈阴性。因此，单克隆抗体114G12在嗅觉受体神经元和犁鼻系统之间做出了分子区分。
