Onoda N, Fujita S C
Department of Physiology, School of Medicine, Gunma University, Japan.
Neuroscience. 1988 Sep;26(3):993-1002. doi: 10.1016/0306-4522(88)90114-5.
Immunohistochemical staining patterns of two monoclonal antibodies produced against the rabbit olfactory bulb were studied in adult rabbit olfactory structures. One monoclonal antibody 112D5 (monoclonal antibody 2D5) stained all of the olfactory receptor cells, whereas the other 114G12 (monoclonal antibody 4G12) stained the upper two-thirds to three-fourths of the receptor cell layer. The negative region in the receptor cell layer was designated the deep compartment. Neither monoclonal antibody stained the supporting cells, basal cells, or Bowman's glands. Monoclonal antibody 2D5 stained the olfactory nerve layer and glomeruli in the olfactory bulb, whereas monoclonal antibody 4G12 stained the whole of the olfactory bulb, particularly the glomeruli and the mitral cells. The piriform cortex was unstained by monoclonal antibody 2D5 whereas the highest immunoreaction to monoclonal antibody 4G12 was in layer Ia. Immunoblot analysis revealed that the molecular weight values of monoclonal antibody 4G12 antigens in the olfactory epithelium were approx. 26,000. Thus, monoclonal antibody 4G12, specific to neurons, recognized an epitope different from the olfactory marker protein specific to the olfactory receptor neurons.
在成年兔嗅觉结构中研究了针对兔嗅球产生的两种单克隆抗体的免疫组织化学染色模式。一种单克隆抗体112D5（单克隆抗体2D5）可染色所有嗅觉受体细胞，而另一种114G12（单克隆抗体4G12）可染色受体细胞层上三分之二至四分之三的区域。受体细胞层中的阴性区域被指定为深部区域。两种单克隆抗体均未染色支持细胞、基底细胞或鲍曼腺。单克隆抗体2D5可染色嗅球中的嗅神经层和肾小球，而单克隆抗体4G12可染色整个嗅球，尤其是肾小球和二尖瓣细胞。梨状皮质未被单克隆抗体2D5染色，而对单克隆抗体4G12的最高免疫反应出现在Ia层。免疫印迹分析显示，嗅觉上皮中单克隆抗体4G12抗原的分子量约为26,000。因此，对神经元具有特异性的单克隆抗体4G12识别的表位与嗅觉受体神经元特有的嗅觉标记蛋白不同。
