Fukami K, Kiyama H, Shiotani Y, Tohyama M
Department of Neuroanatomy, Osaka University Medical School, Japan.
Neuroscience. 1988 Sep;26(3):819-26. doi: 10.1016/0306-4522(88)90102-9.
The neurotensin-containing projections from the retrosplenial cortex to the anterior ventral thalamus were demonstrated by electrolytic lesion studies and fluorescent retrograde tracing combined with immunocytochemistry. Three-to-five-day-old rats were used, because the immunoreactivity of neurotensin fibers in anterior ventral thalamus was the highest at this age. When neurotensin-containing neurons located in layer VI of the retrosplenial cortex were unilaterally destroyed by applying an electrolytic current to the retrosplenial area, the neurotensin fibers in the ipsilateral anterior ventral thalamus decreased dramatically. Unilateral injection of a fluorescent retrograde tracer, Fast Blue, into the anterior ventral thalamus, labeled neurons in the ipsilateral retrosplenial cortex, and many of these cells also had neurotensin-like immunoreactivity. These results suggested that a major origin of the neurotensin fibers in the anterior ventral thalamus was in the ipsilateral retrosplenial granular cortex.
通过电解损伤研究以及荧光逆行追踪与免疫细胞化学相结合的方法，证实了从脾后皮质到腹前丘脑存在含神经降压素的投射。使用3至5日龄的大鼠，因为此时腹前丘脑中神经降压素纤维的免疫反应性最高。当通过向脾后区域施加电解电流单侧破坏位于脾后皮质第VI层的含神经降压素的神经元时，同侧腹前丘脑中的神经降压素纤维显著减少。将荧光逆行示踪剂快蓝单侧注入腹前丘脑，标记了同侧脾后皮质中的神经元，并且这些细胞中的许多也具有神经降压素样免疫反应性。这些结果表明，腹前丘脑中神经降压素纤维的主要起源是同侧脾后颗粒皮质。
