Department of Clinic and Social Dentistry, Federal University of Paraíba, João Pessoa, PB, Brazil.
Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Exp Mol Pathol. 2020 Apr;113:104385. doi: 10.1016/j.yexmp.2020.104385. Epub 2020 Jan 28.
The aim of this study was to assess vascular endothelial growth factor C (VEGF-C) and VEGF-D expressions, tumor lymphatic density (D2-40) and endothelial lymphatic proliferation (D2-40/Ki-67 double labeling) in a series of salivary gland neoplasm cases.
Twenty pleomorphic adenomas (PA), 20 adenoid cystic carcinomas (ACC) and 20 mucoepidermoid carcinomas (MEC) were assessed, as well as 10 normal minor salivary gland (SG) tissues for comparison.
All cases presented positive VEGF-C expression in the peritumoral and intratumoral regions, and no differences in immunoexpression were detected between groups. However, the ACC group presented a significant difference in VEGF-C immunoexpression according to the predominant histological pattern. Most cases presented poor VEGF-D labeling in the peritumoral and intratumoral regions. Concerning peritumoral, intratumoral and total lymphatic endothelial density, the assessed groups revealed an increasing gradient, with lower values for PA, followed by MEC and ACC.
No correlation was detected between VEGF-C and VEGF-D immunoexpression in relation to lymphatic tumor density and endothelial lymphatic proliferation. Western blotting (WB) revealed no difference between VEGF-C and VEGF-D expression among the lesions, corroborating the immunohistochemistry findings.
本研究旨在评估血管内皮生长因子 C（VEGF-C）和 VEGF-D 的表达、肿瘤淋巴管密度（D2-40）以及内皮淋巴管增殖（D2-40/Ki-67 双重标记）在一系列唾液腺肿瘤病例中的情况。
评估了 20 例多形性腺瘤（PA）、20 例腺样囊性癌（ACC）和 20 例黏液表皮样癌（MEC），并选择 10 例正常小唾液腺（SG）组织作为对照。
所有病例在肿瘤周围和肿瘤内区域均呈现阳性的 VEGF-C 表达，各组之间的免疫表达无差异。然而，根据主要组织学模式，ACC 组的 VEGF-C 免疫表达存在显著差异。大多数病例在肿瘤周围和肿瘤内区域的 VEGF-D 标记较差。关于肿瘤周围、肿瘤内和总淋巴管内皮密度，评估的各组呈现出递增的梯度，PA 的值较低，其次是 MEC 和 ACC。
VEGF-C 和 VEGF-D 的免疫表达与淋巴肿瘤密度和内皮淋巴管增殖之间未发现相关性。Western blot（WB）显示病变之间 VEGF-C 和 VEGF-D 的表达无差异，与免疫组化结果相符。
