β,β'-亚氨基二丙腈（IDPN）处理后大鼠坐骨神经轴突细胞骨架重组的形态计量学研究

A morphometric study of cytoskeletal reorganization in rat sciatic nerve axons following beta,beta'-iminodipropionitrile (IDPN) treatment.

作者信息

Nagele R G, Bush K T, Lee H Y

机构信息

Department of Pediatrics, University of Medicine and Dentistry, New Jersey-School of Osteopathic Medicine, Camden 08103.

出版信息

Neurosci Lett. 1988 Oct 17;92(3):241-6. doi: 10.1016/0304-3940(88)90596-4.

DOI:10.1016/0304-3940(88)90596-4

PMID:3200482

Abstract

Electron microscopy and morphometry were used to study changes in the organization of cytoskeletal elements (microtubules (MTs) and neurofilaments (NFs] in myelinated rat sciatic nerve axons resulting from exposure to beta,beta'-iminodipropionitrile (IDPN). Adult male rats were injected subepineurially with IDPN and sacrificed at intervals. Electron micrographs of thin sections through injected nerve segments revealed that MTs segregated from NFs in axons and formed prominent clusters. Morphometric measurements of intermicrotubular distances within these clusters showed that adjacent MTs had a fairly uniform spatial separation that appeared to be maintained by granular MT-associated cross-bridges. In addition, MT clusters underwent a progressive, but reversible, compaction during the first 12 h of IDPN treatment.

摘要

利用电子显微镜和形态测量法研究了成年雄性大鼠坐骨神经有髓轴突中细胞骨架成分（微管（MTs）和神经丝（NFs））在暴露于β，β'-亚氨基二丙腈（IDPN）后组织结构的变化。成年雄性大鼠经神经外膜下注射IDPN，并在不同时间点处死。对注射神经节段的薄切片进行电子显微镜观察发现，轴突中的微管与神经丝分离并形成明显的簇。对这些簇内微管间距离的形态测量表明，相邻微管具有相当均匀的空间间隔，这似乎是由颗粒状微管相关交叉桥维持的。此外，在IDPN处理的最初12小时内，微管簇经历了一个渐进但可逆的压缩过程。