RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA.
Methods Mol Biol. 2020;2113:23-29. doi: 10.1007/978-1-0716-0278-2_3.
Colocalization single-molecule spectroscopy (CoSMoS) allows studying RNA-protein complexes in the full complexity of their cellular environment at single-molecule resolution. Conventionally, the interaction between a single RNA species and multiple proteins is monitored in real time. However, comparing interactions of the same proteins with different RNA species in the same cell extract promises unique insights into RNA biology. Here, we describe an approach to monitor multiple RNA species simultaneously to enable direct comparison. This approach represents a technological development to avoid conventional inter-experiment comparisons.
共定位单分子光谱（CoSMoS）允许在单细胞分辨率下研究其细胞环境中完整复杂性的 RNA-蛋白质复合物。传统上，实时监测单个 RNA 物种与多个蛋白质之间的相互作用。然而，在同一细胞提取物中比较相同蛋白质与不同 RNA 物种的相互作用有望深入了解 RNA 生物学。在这里，我们描述了一种同时监测多种 RNA 物种的方法，以实现直接比较。该方法代表了避免传统实验间比较的技术发展。
