Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China; Department of Microbiology, Government College University, Faisalabad, Pakistan.
Department of Microbiology, Government College University, Faisalabad, Pakistan.
J Glob Antimicrob Resist. 2020 Jun;21:357-362. doi: 10.1016/j.jgar.2020.01.001. Epub 2020 Jan 30.
The rise of carbapenem resistance in Acinetobacter baumannii represents a challenge for the therapeutic management of infections. The present study aimed to investigate the sequence types (STs) and carbapenem resistance in A. baumannii strains collected from various clinical specimens from patients admitted to five tertiary-care hospitals in Pakistan.
A total of 156 A. baumannii clinical strains were analysed for antimicrobial susceptibility, followed by genetic screening for carbapenem resistance determinants. All of the strains were typed by multilocus sequence typing (MLST) according to the Pasteur scheme.
Of the 156 A. baumannii isolates, 139 (89.1%) were carbapenem-resistant, of which 136 carried bla genes. Interestingly, the most commonly identified ST was ST589 (n = 52), classified as clonal complex 1 (CC1). ST2 was the second most common (n = 38), corresponding to CC2/92 (Pasteur/Oxford scheme), which was distributed in all five hospitals.
Diverse clones of carbapenem-resistant A. baumannii, including previously reported STs as well as new STs, carrying bla are distributed in Pakistan. This is the first study to describe the molecular epidemiology of widely disseminated A. baumannii isolates in Pakistan. The findings will help to improve our knowledge of the predominant STs and will be valuable for a deeper understanding of resistance mechanisms among various STs.
鲍曼不动杆菌碳青霉烯类耐药性的上升对感染的治疗管理构成了挑战。本研究旨在调查从巴基斯坦 5 家三级护理医院的各种临床标本中分离的鲍曼不动杆菌菌株的序列类型（ST）和碳青霉烯类耐药性。
对 156 株鲍曼不动杆菌临床菌株进行了抗菌药物敏感性分析，随后进行了碳青霉烯类耐药决定因素的基因筛查。所有菌株均根据巴斯德方案通过多位点序列分型（MLST）进行分型。
在 156 株鲍曼不动杆菌分离株中，有 139 株（89.1%）为碳青霉烯类耐药株，其中 136 株携带 bla 基因。有趣的是，最常见的 ST 是 ST589（n=52），归类为克隆复合体 1（CC1）。其次最常见的 ST 是 ST2（n=38），对应于 CC2/92（巴斯德/牛津方案），分布在所有 5 家医院。
包括先前报道的 ST 以及新的 ST 在内的，携带 bla 的碳青霉烯类耐药鲍曼不动杆菌的多种克隆在巴基斯坦分布。这是首次描述在巴基斯坦广泛传播的鲍曼不动杆菌分离株的分子流行病学。研究结果将有助于提高我们对主要 ST 的了解，并有助于深入了解不同 ST 之间的耐药机制。
