Tze W J, Chen L M
Diabetes. 1977 Mar;26(3):185-91. doi: 10.2337/diab.26.3.185.
Pancreatic islets from adult rats were cultured in artificial capillary cultury units that were perfused with nutrient medium. In this in-vitro culture system, islets remain functional for at least 97 days. As determined by insulin radioimmunoassay of the culture medium, islets continue to secrete insulin. Furthermore, islets responded to high glucose concentration in the perifusion medium by increased insulin release throughout the duration of the culture period. A normal appearance of individual epitheloid islet cells and the presence of aldehyde-fuchsin-positive granules were observed in islets fixed in medium. As indicated by the prolonged maintenance of tissue-specific function, the present simple technique for organ culture of intact islets should be useful for providing new information on the long-term effects in vitro of various factors on islet function and a means for the preservation of islets for transplantation.
将成年大鼠的胰岛培养于灌注有营养培养基的人工毛细管培养单元中。在这个体外培养系统中，胰岛至少能保持功能97天。通过对培养基进行胰岛素放射免疫测定可知，胰岛持续分泌胰岛素。此外，在整个培养期间，胰岛对灌注培养基中高葡萄糖浓度的反应是胰岛素释放增加。在培养基中固定的胰岛中观察到单个类上皮胰岛细胞外观正常且存在醛复红阳性颗粒。鉴于组织特异性功能的长期维持，这种用于完整胰岛器官培养的简单技术应有助于提供关于各种因素对胰岛功能的体外长期影响的新信息，以及作为胰岛移植保存的一种手段。
