Otorhinolaryngology Department, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China; Otorhinolaryngology Department, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510120, China.
The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
J Ethnopharmacol. 2020 Jun 28;256:112638. doi: 10.1016/j.jep.2020.112638. Epub 2020 Jan 31.
Laryngeal carcinoma (LC) is one of the most common malignant head and neck cancers with high incidence and mortality rates. Erchen decoction plus Huiyanzhuyu decoction (EHD) is commonly used for treating LC patients and produces beneficial results. However, the mechanisms underlying the effects of EHD remain unclear.
The present study aimed to analyse the anticancer effects of EHD on the LC cell cycle, apoptosis, migration and invasion in vitro and to explore the underlying biological mechanisms.
TU212 and Hep-2 cells were used. The antitumour effects of EHD were detected by CCK8, microscopy, flow cytometry, EdU incorporation, Hoechst 33342 staining, wound-healing, and transwell assays to assess viability, morphology, apoptosis, cell cycle, migration and invasion, respectively. Furthermore, STAT3 and related proteins were evaluated in laryngeal squamous cell carcinoma (LSCC) cells by Western blot (WB) analysis.
EHD treatment significantly decreased STAT3 and p-STAT3 protein expression levels in LSCC cells. EHD blocked the cell cycle at the G0/G1 phase and induced LSCC apoptosis. Moreover, the viability, migration, and invasion of LSCC cells were markedly inhibited by EHD. In addition, the expression of the cell cycle-related proteins cyclin D1 and cyclin B1 was downregulated in LSCC cells, but P27 expression was increased after EHD treatment. Regarding apoptosis-related proteins, EHD also reduced Bcl-2 expression but upregulated Bax and caspase-3 expression in LSCC cells. In the migration- and invasion-related protein analyses, EHD downregulated MMP-9 expression and upregulated E-cadherin expression.
These results suggest that EHD has an anticancer effect in LSCC. EHD treatment induces apoptosis and inhibits the cell cycle, migration and invasion of LSCC cells, but further work is warranted to address the mechanisms.
喉癌（LC）是最常见的恶性头颈部癌症之一，具有较高的发病率和死亡率。二陈汤加会厌逐瘀汤（EHD）常用于治疗 LC 患者，并取得了良好的效果。然而，EHD 作用的机制尚不清楚。
本研究旨在分析 EHD 对体外 LC 细胞周期、凋亡、迁移和侵袭的抗癌作用，并探讨其潜在的生物学机制。
使用 TU212 和 Hep-2 细胞。通过 CCK8、显微镜、流式细胞术、EdU 掺入、Hoechst 33342 染色、划痕愈合和 Transwell 测定分别评估 EHD 对细胞活力、形态、凋亡、细胞周期、迁移和侵袭的抗肿瘤作用。此外，通过 Western blot（WB）分析评估 STAT3 和相关蛋白在喉鳞癌细胞（LSCC）中的表达。
EHD 处理显著降低了 LSCC 细胞中 STAT3 和 p-STAT3 蛋白的表达水平。EHD 将 LSCC 细胞的细胞周期阻滞在 G0/G1 期，并诱导 LSCC 细胞凋亡。此外，EHD 显著抑制 LSCC 细胞的活力、迁移和侵袭。此外，EHD 还下调了 LSCC 细胞中细胞周期相关蛋白 cyclin D1 和 cyclin B1 的表达，而上调了 P27 的表达。关于凋亡相关蛋白，EHD 还降低了 Bcl-2 的表达，但上调了 Bax 和 caspase-3 的表达。在迁移和侵袭相关蛋白分析中，EHD 下调了 MMP-9 的表达，上调了 E-cadherin 的表达。
这些结果表明 EHD 对 LSCC 具有抗癌作用。EHD 处理诱导凋亡，抑制 LSCC 细胞的细胞周期、迁移和侵袭，但需要进一步的工作来阐明其机制。
