并具有抗淋巴瘤作用。

and anti-lymphoma effects of extract.

机构信息

Department of Basic Medicine and Biomedical Engineering, School of Medicine, Foshan University, Foshan, Guangdong, China.

出版信息

Aging (Albany NY). 2022 May 3;14(9):3801-3812. doi: 10.18632/aging.204041.

DOI:10.18632/aging.204041

PMID:35504024

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9134945/

Abstract

BACKGROUND

Current therapeutic strategies on patients with lymphomas remains limited. Previously we found the suppressive effect of (OPE) on hepatocarcinoma. In present study, the effect of OPE on lymphoma and were investigated.

METHODS

CCK-8 assay was applied to detect the effect of OPE on cell proliferation. Flow cytometry was used to analyze the effect of OPE on cell cycle distribution and apoptosis. Xenograft mouse model was conducted to determine the anti-tumor activity of OPE. TNUEL assay was performed to detect the apoptosis in tumor tissues. Western blot and immuno-histochemistry were used to determine protein expression.

RESULTS

tests indicate that OPE suppressed A20 cell proliferation in a dose- and time-dependent manner. OPE treatment induced cell cycle arrest at S phase and elevated apoptosis in A20 cells. OPE displayed a significant inhibition in tumor growth in a mouse xenograft model. OPE promoted apoptosis of tumor cell in the mouse model Cleaved caspase 3 expression and Bax/Bcl2 ratio were also enhanced. In addition, OPE suppressed A20 cell viability partially by reducing phosphorylation of EGFR.

CONCLUSIONS

Our data showed that OPE suppressed the proliferation of lymphoma cells and promoted apoptosis and , which might be partially mediated by inactivating EGFR signaling.

摘要

背景

目前针对淋巴瘤患者的治疗策略仍然有限。此前我们发现（OPE）对肝癌有抑制作用。在本研究中，我们研究了 OPE 对淋巴瘤和的作用。

方法

CCK-8 法检测 OPE 对细胞增殖的影响。流式细胞术分析 OPE 对细胞周期分布和凋亡的影响。建立异种移植小鼠模型，确定 OPE 的抗肿瘤活性。TUNEL 法检测肿瘤组织中的细胞凋亡。Western blot 和免疫组化法检测蛋白表达。

结果

实验表明，OPE 呈剂量和时间依赖性抑制 A20 细胞增殖。OPE 处理诱导 A20 细胞周期阻滞于 S 期，并促进细胞凋亡。OPE 在小鼠异种移植模型中显著抑制肿瘤生长。OPE 促进肿瘤细胞凋亡，小鼠模型中 Cleaved caspase 3 表达和 Bax/Bcl2 比值增加。此外，OPE 通过降低 EGFR 的磷酸化部分抑制 A20 细胞活力。