College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
Department of Chemistry, Center for Gene Regulation in Health and Diseases, Cleveland State University, Cleveland, OH 44115, USA.
Sci Total Environ. 2020 May 1;715:136953. doi: 10.1016/j.scitotenv.2020.136953. Epub 2020 Jan 25.
This study aims to investigate the neuroprotective effects of 6‴-feruloylspinosin (6-FS), one of the main active flavonoid components in Sour Jujube seeds, on beta-amyloid (Aβ) protein transgenic Caenorhabditis elegans (GMC101) and PC12 cells, and determine the molecular mechanism of its action. We found that 6-FS could ameliorate the progression of the Alzheimer's disease (AD) phenotype by delaying the aging, decreasing the rate of paralysis, enhancing resistance to heat stress, and increasing the chemotaxis ability, and promotes autophagy activity though autophagy/lysosome pathway in GMC101. Furthermore, 6-FS reduced Aβ-induced toxicity by inhibiting the deposition of Aβ and the aggregated proteins, increasing the level of mitophagy in PC12 through promoting the expression of Pink1/Parkin in the mitophagy pathway. Our findings suggest that 6-FS may be used as a medicinal supplement for treating AD.
本研究旨在探讨酸枣仁中主要活性黄酮类成分之一 6‴-阿魏酰斯皮诺素（6-FS）对β-淀粉样蛋白（Aβ）蛋白转基因秀丽隐杆线虫（GMC101）和 PC12 细胞的神经保护作用，并确定其作用的分子机制。我们发现，6-FS 可通过延迟衰老、降低瘫痪率、增强耐热应激能力和增加趋化能力来改善阿尔茨海默病（AD）表型的进展，并通过自噬/溶酶体途径在 GMC101 中促进自噬活性。此外，6-FS 通过抑制 Aβ 的沉积和聚集蛋白的积累，以及通过促进 Pink1/Parkin 在 mitophagy 途径中的表达来增加 PC12 中的 mitophagy 水平，从而减轻 Aβ 诱导的毒性。我们的研究结果表明，6-FS 可作为治疗 AD 的药物补充剂。
