Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Shandong 250022, PR China.
Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Shandong 250022, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Apr 15;231:118115. doi: 10.1016/j.saa.2020.118115. Epub 2020 Jan 25.
Discriminating living and dead cells is of great significance for the study of apoptosis. In this work, we have developed a unique fluorescent probe (RPIC) for discriminating live and dead cells with duel-channel fluorescence imaging under double excitation and double emission mode. Dead cells treated with RPIC shows weak fluorescence signals in red channel, however, strong fluorescence signals are appeared in red channel in live cells. Weak and strong green fluorescence signals present at live cells and dead cells, respectively. Moreover, RPIC can detect successfully apoptosis of cancer cells. For in-vivo imaging, RPIC can discriminate successfully live and dead zebrafish with the same method. More interestingly, it is found that RPIC possesses the ability of discriminating normal mice and tumor mice.
区分活细胞和死细胞对于细胞凋亡的研究具有重要意义。在这项工作中，我们开发了一种独特的荧光探针（RPIC），用于在双激发和双发射模式下进行双通道荧光成像，以区分活细胞和死细胞。用 RPIC 处理的死细胞在红色通道中显示出较弱的荧光信号，而活细胞在红色通道中则显示出较强的荧光信号。弱和强的绿色荧光信号分别出现在活细胞和死细胞中。此外，RPIC 可以成功检测癌细胞的凋亡。对于体内成像，RPIC 可以使用相同的方法成功区分活鱼和死鱼。更有趣的是，发现 RPIC 具有区分正常小鼠和肿瘤小鼠的能力。
