Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, P. R. China.
Analyst. 2019 Oct 22;144(21):6247-6253. doi: 10.1039/c9an01573f.
Intracellular viscosity abnormalities can lead to diabetes, neurodegenerative diseases and cancer. In this work, we developed a mitochondria-targetable fluorescent probe (EIMV) for discriminating normal and inflammatory models by viscosity changes. It was found that EIMV showed excellent properties, including high photostability, low cytotoxicity, red emission and favorable biocompatibility. In view of these unique features, this probe could successfully identify normal and cancer cells via viscosity changes. Furthermore, the EIMV probe successfully identified zebrafish with different viscosities by the same method. Moreover, EIMV exhibited different fluorescence signals in normal and inflammatory mice due to changes in viscosity. Therefore, the probe provides a new method to study the relationship between diseases and viscosity in the fields of biology and medicine.
细胞内粘度异常可导致糖尿病、神经退行性疾病和癌症。在这项工作中，我们开发了一种线粒体靶向荧光探针（EIMV），可通过粘度变化来区分正常和炎症模型。研究发现，EIMV 表现出优异的性能，包括高稳定性、低细胞毒性、红色发射和良好的生物相容性。鉴于这些独特的特性，该探针可以通过粘度变化成功识别正常和癌细胞。此外，该探针还可以通过相同的方法成功识别出具有不同粘度的斑马鱼。此外，由于粘度的变化，EIMV 在正常和炎症小鼠中表现出不同的荧光信号。因此，该探针为研究生物学和医学领域疾病与粘度之间的关系提供了一种新方法。
