用于 100%水溶液中次氯酸盐的灵敏和选择性荧光探针及其用于溶酶体靶向细胞成像的应用。
Sensitive and selective fluorescent probe for hypochlorite in 100% aqueous solution and its application for lysosome-targetable cell imaging.
机构信息
College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China.
College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Apr 15;231:118110. doi: 10.1016/j.saa.2020.118110. Epub 2020 Jan 25.
A morpholine-functionalized pyrrole-cyanine probe was synthesized via a simple condensation reaction in high yield. This probe exhibits high selectivity toward ClO on fluorescence and UV-vis spectra in neat aqueous solution. The strong green emission of the probe solution was quenched and the yellow color faded immediately upon the addition of ClO. The detection limit of the probe for ClO was 0.165 μM. The mechanism of hypochlorite-induced CC breakage was supposed on the basis of EIS-MS, NMR, and density functional theory (DFT) calculation. Finally, the probe was utilized to image ClO in lysosomes of living cells.
一种吗啉基功能化吡咯菁探针通过简单的缩合反应高产率合成。该探针在纯水溶液中对 ClO 表现出高选择性的荧光和紫外可见光谱。探针溶液的强绿色发射被猝灭，并且在加入 ClO 后黄色立即褪色。该探针对 ClO 的检测限为 0.165 μM。基于电化学阻抗谱、NMR 和密度泛函理论（DFT）计算，推测了次氯酸盐诱导的 CC 断裂的机制。最后，该探针被用于在活细胞的溶酶体中成像 ClO。
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