用于活细胞中次氯酸检测的溶酶体靶向双光子荧光探针。
Lysosomal-Targeted Two-Photon Fluorescent Probe to Sense Hypochlorous Acid in Live Cells.
机构信息
Phosphorus Chemical Engineering Research Center of Henan Province, The College of Chemistry and Molecular Engineering, Zhengzhou University , Zhengzhou 450001, China.
Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Henan Province, School of Pharmaceutical Sciences, Zhengzhou University , Zhengzhou 450001, Henan China.
出版信息
Anal Chem. 2017 Oct 3;89(19):10384-10390. doi: 10.1021/acs.analchem.7b02361. Epub 2017 Sep 14.
A two-photon reversible fluorescent probe L1 was designed and synthesized. The fluorescence intensity of the probe solution was strong, while the fluorescence of the solution was obviously quenched and the color of the solution was changed after the addition of hypochlorous acid, indicating this is "naked-eye sensor" for the detection of HClO. The probe showed great selectivity for hypochlorous acid over other reactive oxygen species (ROS) and anions. Fluorescence titration experiments showed that the probe has a low detection limit of 0.674 μM. Because of a morpholine group introduced to the naphathalimide framework, probe L1 was successfully applied to detect intracellular HClO in lysosome.
设计并合成了一种双光子可逆荧光探针 L1。探针溶液的荧光强度较强，而加入次氯酸后，探针溶液的荧光明显猝灭，溶液颜色发生变化，表明这是检测 HClO 的“肉眼传感器”。该探针对次氯酸具有很强的选择性，优于其他活性氧（ROS）和阴离子。荧光滴定实验表明，该探针的检测限低至 0.674 μM。由于在萘酰亚胺骨架中引入了吗啉基团，探针 L1 成功地应用于溶酶体中检测细胞内 HClO。
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