Division of Biosafety control and Research, National Institute of Infectious Diseases.
YONAGO Medical Center, National Hospital Organization.
Jpn J Infect Dis. 2020 May 22;73(3):253-255. doi: 10.7883/yoken.JJID.2019.122. Epub 2020 Jan 31.
Considering the possibility that Escherichia coli carried by companion dogs could infect owners and human society, we investigated their pathogenicity and drug resistance. E. coli was isolated from stool samples of companion dogs (n = 90) to examine the O-serogroup, virulence genes, and drug susceptibility. The age of dogs ranged from 4 months to 16 years, and they were mainly treated with cefalexin, enrofloxacin, or amoxicillin. A total of 69 samples were positive for E. coli (76% of examined dogs), and the most common O-serogroup was O18 (n = 13). Nine diarrheagenic E. coli, including enteropathogenic E. coli (n = 3), enteroaggregative E. coli (n = 1), and astA-carrying E. coli (n = 5), were isolated. In addition, we isolated 28 E. coli strains resistant to at least one of six antimicrobials, including cephalothin (CET), ceftazidime (CAZ), cefotaxime (CTX), chloramphenicol (CP), fosfomycin (FOM), and norfloxacin (NLFX). The resistance pattern was as follows: CET, n = 16; NLFX, n = 3; CET/CP (resistance to both CET and CP), n = 1; CET/NLFX, n = 1; CET/CAZ/CTX, n = 3; CET/CTX/NLFX, n = 2; CET/CP/NLFX, n = 1; and CET/CAZ/CTX/NLFX, n = 1. Moreover, ten E. coli isolates were found to produce extended-spectrum β-lactamase (ESBL), including AmpC (n = 4; OUT, O18, O74, and O166), CTX-M-1 (n = 1; O25), CTX-M-9 (n = 4; OUT, O18, O18, and O125), and AmpC/CTX-M-9 (n = 1; OUT) groups. The AmpC-producing E. coli strains included enteropathogenic and astA-carrying E. coli. Our results showed that the human-infectious diarrheagenic E. coli was isolated from some dogs, and some strains exhibited ESBL. Therefore, future studies are needed to investigate the possibility of transmission of these E. coli strains to humans.
考虑到伴侣犬携带的大肠杆菌可能感染主人和人类社会的可能性，我们对其致病性和耐药性进行了研究。我们从 90 只伴侣犬的粪便样本中分离出大肠杆菌，以检查其 O-血清群、毒力基因和药敏情况。犬的年龄从 4 个月到 16 岁不等，主要接受头孢氨苄、恩诺沙星或阿莫西林治疗。共有 69 份样本检测出大肠杆菌阳性（被检犬的 76%），最常见的 O-血清群为 O18（n = 13）。分离出 9 株致泻性大肠杆菌，包括肠致病性大肠杆菌（n = 3）、肠聚集性大肠杆菌（n = 1）和携带 astA 的大肠杆菌（n = 5）。此外，我们还分离出 28 株至少对 6 种抗菌药物中的 1 种耐药的大肠杆菌，包括头孢噻肟（CET）、头孢他啶（CAZ）、头孢噻肟（CTX）、氯霉素（CP）、磷霉素（FOM）和诺氟沙星（NLFX）。耐药模式如下：CET，n = 16；NLFX，n = 3；CET/CP（对 CET 和 CP 均耐药），n = 1；CET/NLFX，n = 1；CET/CAZ/CTX，n = 3；CET/CTX/NLFX，n = 2；CET/CP/NLFX，n = 1；以及 CET/CAZ/CTX/NLFX，n = 1。此外，10 株大肠杆菌被发现产生超广谱β-内酰胺酶（ESBL），包括 AmpC（n = 4；OUT、O18、O74 和 O166）、CTX-M-1（n = 1；O25）、CTX-M-9（n = 4；OUT、O18、O18 和 O125）和 AmpC/CTX-M-9（n = 1；OUT）组。产 AmpC 的大肠杆菌菌株包括肠致病性和携带 astA 的大肠杆菌。我们的结果表明，一些狗中分离出了可感染人类的致泻性大肠杆菌，一些菌株表现出 ESBL。因此，未来需要研究这些大肠杆菌菌株向人类传播的可能性。
