犬猫中产超广谱β-内酰胺酶细菌的全球流行率及分子特征——一项范围综述和荟萃分析

Global prevalence and molecular characterization of extended-spectrum β-lactamase producing- in dogs and cats - A scoping review and meta-analysis.

作者信息

Salgado-Caxito Marília, Benavides Julio A, Adell Aiko D, Paes Antonio Carlos, Moreno-Switt Andrea I

机构信息

Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.

Millennium Initiative for Collaborative Research On Bacterial Resistance (MICROB-R), Santiago, Chile.

出版信息

One Health. 2021 Mar 20;12:100236. doi: 10.1016/j.onehlt.2021.100236. eCollection 2021 Jun.

Antimicrobial resistance (AMR) represents a major threat to human and animal health. Part of the AMR dimension is the circulation of extended-spectrum β-lactamases producing- (ESBL-), which is now commonly reported among companion animals. However, the global perspective of the prevalence and population structure of ESBL- circulating in dogs and cats has not been estimated limiting our understanding of their role in the dissemination of ESBL-. The aim of this study was to compare the prevalence of ESBL- between dogs and cats and across countries through meta-analysis. We also performed a scoping review to summarize the current knowledge on ESBL genes and clones circulating among companion animals. A total of 128 studies published in PubMed, Web of Science, and Scopus up to April 2020 were selected and contained information on prevalence and/or molecular characterization of ESBL genes and ESBL- clones. Our review shows an increase in the number of publications between 2000 and 2019, concentrated mainly in Europe. Prevalence varied across continents, ranging from 0.63% (Oceania) to 16.56% (Africa) in dogs and from 0% (Oceania) to 16.82% (Asia) in cats. Although there were twice as many studies reporting prevalence on dogs ( = 61) than on cats ( = 32), and only 9 studies focused exclusively on cats, our meta-analysis showed no difference in the global prevalence of ESBL- between dogs (6.87% [95% CI: 4.46-10.45%]) and cats (5.04% [95% CI: 2.42-10.22%]). A considerable diversity of ESBL genes ( = 60) and sequence types (ST) ( = 171) were recovered from companion animals. ESBL- encoded by CTX-M-15 (67.5%, 77/114) and SHV-12 (21.9%, 25/114), along with resistant strains of ST38 (22.7%, 15/66) and ST131 (50%, 33/66) were widespread and detected in all continents. While presence of ESBL- is widespread, the drivers influencing the observed ESBL- prevalence and the clinical relevance in veterinary medicine and public health along with economic impact of ESBL- infections among companion animals need to be further investigated.

抗菌药物耐药性（AMR）对人类和动物健康构成重大威胁。AMR的一个方面是产超广谱β-内酰胺酶（ESBL）细菌的传播，目前在伴侣动物中普遍有报道。然而，在全球范围内，犬猫体内ESBL的流行情况和种群结构尚未得到评估，这限制了我们对其在ESBL传播中作用的理解。本研究的目的是通过荟萃分析比较犬猫之间以及不同国家的ESBL流行情况。我们还进行了一项范围综述，以总结目前关于伴侣动物中ESBL基因和克隆的知识。总共筛选了截至2020年4月发表在PubMed、Web of Science和Scopus上的128项研究，这些研究包含ESBL基因和ESBL克隆的流行情况和/或分子特征信息。我们的综述显示，2000年至2019年间发表的文献数量有所增加，主要集中在欧洲。各大洲的流行率各不相同，犬的流行率从0.63%（大洋洲）到16.56%（非洲），猫的流行率从0%（大洋洲）到16.82%（亚洲）。虽然报告犬ESBL流行情况的研究（n = 61）是猫（n = 32）的两倍，且仅有9项研究专门针对猫，但我们的荟萃分析显示，全球犬（6.87% [95% CI：4.46 - 10.45%]）和猫（5.04% [95% CI：2.42 - 10.22%]）的ESBL流行率没有差异。从伴侣动物中发现了相当多样的ESBL基因（n = 60）和序列类型（ST）（n = 171）。由CTX-M-15（67.5%，77/114）和SHV-12（21.9%，25/114）编码的ESBL，以及ST38（22.7%，15/66）和ST131（50%，33/66）的耐药菌株广泛存在且在各大洲均有检测到。虽然ESBL的存在很普遍，但影响观察到的ESBL流行率的驱动因素、其在兽医学和公共卫生中的临床相关性以及伴侣动物中ESBL感染的经济影响仍需进一步研究。