Department of Radiologic Diagnosis, Fujian Cancer Hospital & Fujian Medical University Cancer Hospital, Fuzhou, Fujian, China.
Department of Radiation Oncology, Fujian Cancer Hospital & Fujian Medical University Cancer Hospital, Fuzhou, Fujian, China.
Jpn J Clin Oncol. 2020 May 5;50(5):548-555. doi: 10.1093/jjco/hyaa002.
To investigate the correlations and feasibility of diffusion kurtosis imaging (DKI) parameters and tumour histopathology after radiotherapy in human nasopharyngeal carcinoma (NPC) xenografts on nude mice.
Seventy-two nude mice were used for the construction of CNE-1 (radio-insensitive) and CNE-2 (radio-sensitive) NPC xenograft models, followed by fraction irradiation at different doses of X-ray. The nude mice were randomly divided into six groups in each cell line models according to the dose of X-ray they have received and with six mice in each group. DKI scan was performed after radiation. DKI parameters, tumour histopathology and AQP-1 biomarkers were detected. One-way ANOVA and Pearson's correlation analysis were used in statistical analysis.
In CNE-1 and CNE-2 NPC xenografts, D values were increased (P < 0.01 and P < 0.001), while K values (P < 0.01 and P < 0.001) and tumour size (P < 0.001 and P < 0.001) were reduced during fraction irradiation. Additionally, cell density (CD) and AQP-1 expressions were decreased, and necrosis ratio (NR) was increased in CNE-2 xenografts after fraction irradiation (P < 0.001). The changes in D values were negatively correlated with tumour size (r = -0.856, P < 0.001), CD (r = -0.918, P < 0.001), AQP-1 mRNA (r = -0.856, P < 0.001) and protein (r = -0.381, P = 0.022) expressions while positively correlated with NR (r = 0.908, P < 0.001) in CNE-2 xenografts. The changes in K values were positively correlated with tumour size (r = 0.964, P < 0.001), CD (r = 0.888, P < 0.001), AQP-1 mRNA (r = 0.955, P < 0.001) and protein (r = 0.330, P = 0.049) expression levels while negatively correlated with NR (r = -0.930, P < 0.001). However, in CNE-1 xenografts, there were no correlation between DKI parameters and the expression of AQP-1.
Changes in D and K parameters after fractional irradiation are closely related with NPC cellular and pathological characteristics, especially size reduction and necrosis induction. These parameters exhibit potential abilities of monitoring the response to fractional irradiation in radio-sensitive NPC xenografts.
探讨扩散峰度成像（DKI）参数与荷人鼻咽癌细胞（NPC）裸鼠移植瘤经分割照射后肿瘤组织病理学变化的相关性和可行性。
构建 72 只裸鼠鼻咽癌细胞 CNE-1（放射抗拒）和 CNE-2（放射敏感）移植瘤模型，随后给予不同剂量 X 射线分割照射。根据接受的 X 射线剂量，将裸鼠在两种细胞系模型中随机分为 6 组，每组 6 只。照射后行 DKI 扫描。检测 DKI 参数、肿瘤组织病理学和水通道蛋白-1（AQP-1）标志物。采用单因素方差分析和 Pearson 相关性分析进行统计学分析。
在 CNE-1 和 CNE-2 NPC 移植瘤中，随着分割照射的进行，D 值增加（P<0.01 和 P<0.001），而 K 值（P<0.01 和 P<0.001）和肿瘤体积（P<0.001 和 P<0.001）减小。此外，CNE-2 移植瘤的细胞密度（CD）和 AQP-1 表达减少，坏死率（NR）增加（P<0.001）。CNE-2 移植瘤中 D 值的变化与肿瘤体积（r=-0.856，P<0.001）、CD（r=-0.918，P<0.001）、AQP-1 mRNA（r=-0.856，P<0.001）和蛋白（r=-0.381，P=0.022）表达呈负相关，与 NR（r=0.908，P<0.001）呈正相关。K 值的变化与肿瘤体积（r=0.964，P<0.001）、CD（r=0.888，P<0.001）、AQP-1 mRNA（r=0.955，P<0.001）和蛋白（r=0.330，P=0.049）表达呈正相关，与 NR（r=-0.930，P<0.001）呈负相关。然而，在 CNE-1 移植瘤中，DKI 参数与 AQP-1 表达之间无相关性。
分割照射后 D 和 K 参数的变化与 NPC 细胞和病理特征密切相关，特别是肿瘤体积缩小和坏死诱导。这些参数在监测放射敏感 NPC 移植瘤对分割照射的反应方面具有潜在的能力。
