Zelt R G, Daniel R K, Ballard P A, Brissette Y, Heroux P
Division of Plastic and Reconstructive Surgery, Royal Victoria Hospital, Montreal, Quebec, Canada.
Plast Reconstr Surg. 1988 Dec;82(6):1027-41. doi: 10.1097/00006534-198812000-00014.
A chronic electrical burn model employing documentary and diagnostic techniques was designed in the primate for investigating wound evolution up to 10 days after injury. A standardized 40-kJ, 3500-V, 4.2-A, 2.5-s bilateral, symmetrical upper extremity electrical injury was performed. Gross observation studies documented tissue injury extending more proximally on the deep surfaces of individual muscles and between muscle layers. Specific regions, or "choke" points, in the forearm exist in which decreased cross-sectional areas and highly resistant tissue composition resulted in increased heat production and more severe tissue damage. Muscle injury was analyzed using light microscopy, revealing patchy cellular necrosis intermixed with viable cells. Digital subtraction angiography demonstrated segmental narrowing and "pruning" of large vascular trunks with a significant decrease in nutrient vessels in affected areas. Ulnar nerve conduction studies showed loss of conduction proximal to the cubital fossa with no recovery. Although characteristic patterns of injury were documented in skin, muscle, vessels, and nerves, no experimental evidence was found for progressive necrosis.
设计了一种采用记录和诊断技术的慢性电烧伤模型，用于在灵长类动物中研究损伤后长达10天的伤口演变。进行了标准化的40千焦、3500伏、4.2安、2.5秒的双侧对称上肢电损伤。大体观察研究记录了组织损伤在个体肌肉深层表面和肌层之间向近端延伸的情况。在前臂存在特定区域或“阻塞”点，其中横截面积减小和组织成分高度抗性导致产热增加和更严重的组织损伤。使用光学显微镜分析肌肉损伤，显示散在的细胞坏死与存活细胞混合。数字减影血管造影显示大血管主干节段性狭窄和“修剪”，受影响区域的营养血管显著减少。尺神经传导研究显示肘窝近端传导丧失且无恢复。尽管在皮肤、肌肉、血管和神经中记录了特征性的损伤模式，但未发现进行性坏死的实验证据。
