放射免疫测定法和双位点免疫放射“夹心”测定法：基本原理。

Radioimmunoassays and 2-site immunoradiometric "sandwich" assays: basic principles.

作者信息

Rodbard D

机构信息

Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892.

出版信息

Radioisotopes. 1988 Oct;37(10):590-4. doi: 10.3769/radioisotopes.37.10_590.

DOI:10.3769/radioisotopes.37.10_590

PMID:3201008

Abstract

The "sandwich" or noncompetitive reagent-excess, 2-site immunoradiometric assay (2-site IRMA), ELISA, USERIA, and related techniques, have several advantages compared with the traditional or competitive radioimmunoassays. IRMAs can provide improved sensitivity and specificity. However, IRMAs present some practical problems with nonspecific binding, increased consumption of antibody, biphasic dose response curve, (high dose hook effect), and may require special techniques for dose response curve analysis. We anticipate considerable growth in the popularity and importance of 2-site IRMA.

摘要

“夹心”或非竞争性试剂过量双位点免疫放射分析（2 位点 IRMA）、酶联免疫吸附测定（ELISA）、用户免疫放射分析（USERIA）及相关技术，与传统的或竞争性放射免疫分析相比具有若干优势。免疫放射分析可提高灵敏度和特异性。然而，免疫放射分析存在一些实际问题，如非特异性结合、抗体消耗增加、双相剂量反应曲线（高剂量钩效应），并且可能需要特殊技术来分析剂量反应曲线。我们预计双位点免疫放射分析的普及程度和重要性将大幅增长。