Miyachi Y
1st Dept. of Int. Med., Toho Univ. Sch. of Med.
Nihon Rinsho. 1995 Sep;53(9):2112-8.
Radioimmunoassays are simple and quite useful, being easily applied on a commercially serviceable basis. Furthermore radioimmunoassays are extremely sensitive and quite precise. Two site immunoradiometric assay in which one antibody was immobilized and another labelled with 125I becomes gradually popular, due to its higher sensitivity and the development of monoclonal antibody production by the hybridoma technique. Alternative analytical methods other than radioimmunoassays (radioimmunoassay, immunoradiometric assay) have been developed, in which the alternative labels to substitute for radioisotopes are enzymes, luminescent compounds and fluorescent probes.
放射免疫测定法简单且非常有用,易于在商业可用的基础上应用。此外,放射免疫测定法极其灵敏且相当精确。一种抗体固定化而另一种用125I标记的双位点免疫放射测定法,由于其更高的灵敏度以及通过杂交瘤技术生产单克隆抗体的发展,正逐渐流行起来。除了放射免疫测定法(放射免疫分析、免疫放射测定法)之外,还开发了其他分析方法,其中替代放射性同位素的替代标记物是酶、发光化合物和荧光探针。