Teimoori-Boghsani Yeganeh, Ganjeali Ali, Cernava Tomislav, Müller Henry, Asili Javad, Berg Gabriele
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Institute of Environmental Biotechnology, Graz University of Technology, Graz, Austria.
Front Microbiol. 2020 Jan 17;10:3013. doi: 10.3389/fmicb.2019.03013. eCollection 2019.
Endophytic fungi are often embedded in their host's metabolic networks, which can result in alterations of metabolite production and higher amounts of active compounds in medicinal plants. This study reports the occurrence, diversity, and secondary metabolite profiles of endophytic fungi isolated from plants obtained from three geographically distinct sites in Iran. A total of 56 endophytic fungi were isolated from roots and leaves of . site-specificity and root-dominated colonization was found to be a general characteristic of the endophytes. Based on molecular identification, the endophytic fungi were classified into 15 genera. Mycelial extracts of these isolates were subjected to high-resolution mass spectrometry analyses and revealed a broad spectrum of secondary metabolites. Our results demonstrated that , , , and are producers of cryptotanshinone, which is a main bioactive compound of . . Moreover, it was shown that it can be produced independent of the host plant. The effect of exogenous gibberellin on and endophytic fungi was shown to have a positive effect on increasing the cryptotanshinone production in the plant as well as in endophytic fungi cultivated under axenic conditions. Our findings provide further evidence that endophytic fungi play an important role in the production plant bioactive metabolites. Moreover, they provide an exploitable basis to increase cryptotanshinone production in . .
内生真菌常常嵌入其宿主的代谢网络中，这可能导致药用植物中代谢物产生的改变以及活性化合物含量的增加。本研究报告了从伊朗三个地理位置不同的地点采集的植物中分离出的内生真菌的发生情况、多样性和次生代谢产物谱。总共从[植物名称]的根和叶中分离出56株内生真菌。发现位点特异性和根主导的定殖是内生真菌的一个普遍特征。基于分子鉴定，这些内生真菌被分为15个属。对这些分离物的菌丝体提取物进行了高分辨率质谱分析，揭示了广泛的次生代谢产物。我们的结果表明，[真菌名称1]、[真菌名称2]、[真菌名称3]和[真菌名称4]是隐丹参酮的生产者，隐丹参酮是[植物名称]的主要生物活性化合物。此外，研究表明它可以独立于宿主植物产生。外源赤霉素对[植物名称]和内生真菌的作用显示出对增加植物以及在无菌条件下培养的内生真菌中隐丹参酮产量具有积极影响。我们的研究结果提供了进一步的证据，证明内生真菌在植物生物活性代谢产物的产生中起重要作用。此外，它们为增加[植物名称]中隐丹参酮的产量提供了可利用的基础。
