Völkel Sabrina, Hein Sascha, Benker Nathalie, Pfeifer Felicitas, Lenz Christof, Losensky Gerald
Microbiology and Archaea, Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany.
Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany.
Front Microbiol. 2020 Jan 17;10:3056. doi: 10.3389/fmicb.2019.03056. eCollection 2019.
R1 is an extremely halophilic archaeon capable of adhesion and forming biofilms, allowing it to adjust to a range of growth conditions. We have recently shown that living in biofilms facilitates its survival under Cu and Ni stress, with specific rearrangements of the biofilm architecture observed following exposition. In this study, quantitative analyses were performed by SWATH mass spectrometry to determine the respective proteomes of planktonic and biofilm cells after exposition to Cu and Ni.Quantitative data for 1180 proteins were obtained, corresponding to 46% of the predicted proteome. In planktonic cells, 234 of 1180 proteins showed significant abundance changes after metal ion treatment, of which 47% occurred in Cu and Ni treated samples. In biofilms, significant changes were detected for 52 proteins. Only three proteins changed under both conditions, suggesting metal-specific stress responses in biofilms. Deletion strains were generated to assess the potential role of selected target genes. Strongest effects were observed for ΔOE5245F and ΔOE2816F strains which exhibited increased and decreased biofilm mass after Ni exposure, respectively. Moreover, EPS obviously plays a crucial role in metal ion resistance. Further efforts are required to elucidate the molecular basis and interplay of additional resistance mechanisms.
R1是一种极端嗜盐古菌，能够粘附并形成生物膜，使其能够适应一系列生长条件。我们最近发现，生活在生物膜中有助于其在铜和镍胁迫下存活，暴露后观察到生物膜结构有特定的重排。在这项研究中，通过SWATH质谱法进行定量分析，以确定暴露于铜和镍后浮游细胞和生物膜细胞的各自蛋白质组。获得了1180种蛋白质的定量数据，相当于预测蛋白质组的46%。在浮游细胞中，1180种蛋白质中有234种在金属离子处理后显示出显著的丰度变化，其中47%发生在铜和镍处理的样品中。在生物膜中，检测到52种蛋白质有显著变化。只有三种蛋白质在两种条件下都发生了变化，表明生物膜中存在金属特异性应激反应。构建了缺失菌株以评估选定靶基因的潜在作用。对ΔOE5245F和ΔOE2816F菌株观察到最强的效应，它们在镍暴露后分别表现出生物膜质量增加和减少。此外，胞外聚合物显然在金属离子抗性中起关键作用。需要进一步努力阐明其他抗性机制的分子基础和相互作用。
