肝囊型包虫病患者中转化生长因子-β1与肝纤维化的相关性研究
Study on the association between TGF-β1 and liver fibrosis in patients with hepatic cystic echinococcosis.
作者信息
Tian Fengming, Liu Yumei, Gao Jian, Yang Ning, Shang Xiaoqian, Lv Jie, Ba Derong, Zhou Xuan, Zhang Chuntao, Ma Xiumin
机构信息
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Medical Research Institute of First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
College of Basic Medicine of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
出版信息
Exp Ther Med. 2020 Feb;19(2):1275-1280. doi: 10.3892/etm.2019.8355. Epub 2019 Dec 19.
The present study aimed to determine the role of the cytokine transforming growth factor-β1 (TGF-β1) in liver fibrosis among patients with hepatic cystic echinococcosis (hepatic CE). Hepatic tissue specimens and serum samples from 30 patients with hepatic CE were collected and TGF-β1 levels were compared between the two groups. The degree of liver fibrosis was assessed by Masson staining. The expression levels of cytokine TGF-β1 in liver tissue and serum were detected by immunohistochemistry and ELISA, respectively. Masson staining of liver lesion tissue in patients with hepatic CE indicated different degrees of fibrosis in the liver and the World Health Organization classification was positively correlated with the severity of liver fibrosis (P<0.05). In addition, the expression of cytokine TGF-β1 was higher in liver lesion tissue specimens compared with that in the adjacent control samples (P<0.05). At the same time, cytokine TGF-β1 in serum specimens of patients was higher than that in the healthy control group (P<0.05). In conclusion, the expression of TGF-β1 is upregulated in patients with hepatic CE, which was closely associated to liver fibrosis.
本研究旨在确定细胞因子转化生长因子-β1（TGF-β1）在肝囊性棘球蚴病（肝CE）患者肝纤维化中的作用。收集30例肝CE患者的肝组织标本和血清样本，比较两组之间的TGF-β1水平。采用Masson染色评估肝纤维化程度。分别通过免疫组织化学和ELISA检测肝组织和血清中细胞因子TGF-β1的表达水平。肝CE患者肝病变组织的Masson染色显示肝脏存在不同程度的纤维化，且世界卫生组织分类与肝纤维化严重程度呈正相关（P<0.05）。此外，与相邻对照样本相比，肝病变组织标本中细胞因子TGF-β1的表达更高（P<0.05）。同时，患者血清标本中的细胞因子TGF-β1高于健康对照组（P<0.05）。总之，肝CE患者中TGF-β1的表达上调，这与肝纤维化密切相关。
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