Saftawy E A El, Abdelraouf A, Elsalam M A, Zakareya P, Fouad A, Albadawi E A, Abobakr Ali A H S, Amin N M
Medical Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Medical Parasitology Department, Faculty of Medicine, Armed Forces College of Medicine, Cairo, Egypt.
Helminthologia. 2022 May 4;59(1):1-17. doi: 10.2478/helm-2022-0011. eCollection 2022 Mar.
Cystic echinococcosis is speculated to exert several immune-evasion strategies involving autoimmune-phenomena. We evaluated the hypothesizes that the prevalence of autoantibodies increases in the sera of CE patients that may evidence the association between the parasite and autoimmune diseases. Sera from 63 subjects at distinct types of CE cyst fertility were investigated for antinuclear antibodies (ANA), and anti-CCP antibodies. Plasma levels and cellular production of IL-17A cytokine were specifically defined as being assumed to prime for autoimmunity. Healthy-controls were age and gender-matched to test sera. ANA expressions inside the surgically removed metacestode and adventitial layer were also assayed. Out of 63 patients, 35 % had fertile highly viable cysts (group-1), 41 % had fertile low viable cysts (group-2) and 24 % had non-fertile cysts (group-3). A four-fold increase in ANA sera-levels was detected in group-1 compared with their controls (value 0.001) while anti-CCP levels were of insignificant differences. In group-2 and group-3, no significant differences were detected between ANA and anti-CCP sera-levels in CE patients and their controls. IL-17A sera-levels in group-1 and group- 2 were significantly higher than their healthy-controls while being of insignificant differences in group-3, value= 0.300. No association was detected between sera-levels of IL-17A and ANA as well as anti-CCP antibodies. Interestingly, relative IL-17A cellular expression associated positive ANA deposition in the parasite cells and adventitial layer. Collectively, based on the parasite fertility, IL-17A and ANA seemed to be involved in the host immune defenses against CE. There is no association between CE and anti-CCP antibodies.
推测囊性棘球蚴病会采用多种涉及自身免疫现象的免疫逃避策略。我们评估了以下假设：在囊性棘球蚴病（CE）患者血清中自身抗体的患病率会增加，这可能证明寄生虫与自身免疫性疾病之间存在关联。对63名处于不同类型CE囊肿繁殖力阶段的受试者的血清进行了抗核抗体（ANA）和抗环瓜氨酸肽（CCP）抗体检测。白细胞介素-17A（IL-17A）细胞因子的血浆水平和细胞产生被明确认为可能引发自身免疫。健康对照在年龄和性别上与测试血清匹配。还检测了手术切除的囊尾蚴和外膜层内的ANA表达。63名患者中，35%有繁殖力高且活力强的囊肿（第1组），41%有繁殖力低且活力低的囊肿（第2组），24%有非繁殖性囊肿（第3组）。与对照组相比，第1组患者血清ANA水平增加了四倍（值为0.001），而抗CCP水平差异不显著。在第2组和第3组中，CE患者及其对照组的ANA和抗CCP血清水平未检测到显著差异。第1组和第2组患者的血清IL-17A水平显著高于健康对照组，而第3组差异不显著（值=0.300）。未检测到IL-17A血清水平与ANA以及抗CCP抗体之间存在关联。有趣的是，相对IL-17A细胞表达与寄生虫细胞和外膜层中ANA阳性沉积相关。总体而言，基于寄生虫的繁殖力，IL-17A和ANA似乎参与了宿主针对CE的免疫防御。CE与抗CCP抗体之间没有关联。
