Shamas S, Rani S, Afsheen S, Shahab M, Ejaz R, Sadia H, Khan L, Rehman T U, Roshan S, Mayo A
University of Gujrat - Hafiz Hayat Campus - Department of Zoology, Gujrat, Pakistan.
Quaid-i-Azam University - Animal Sciences, Islamabad, Pakistan.
Acta Endocrinol (Buchar). 2019 Jul-Sep;15(3):283-288. doi: 10.4183/aeb.2019.283.
Kisspeptin role in metabolism has been implicated recently. However, the nature of the signals that may connect body fat/muscle tissues with the central nervous system governing energy homeostasis remains to be elucidated.
The present study was designed to investigate the effects of peripheral kisspeptin-10 administration on irisin release in human males.
Kisspeptin-10 was administered to normal weight (n=8) and obese (n=8) men. Sequential blood sampling was performed for 30 minutes pre and 210 minutes post kisspeptin injection at 30 minutes interval. ELISA kit was used to detect plasma irisin levels.
There is a significant (P<0.0001) effect of Kisspeptin-10 administration on irisin release in both normal weight and obese participants. Mean irisin levels (96.24 ± 1.351 ng/mL) at 210 minutes were significantly (P<0.0001) enhanced as compared to pre-kisspeptin (59.18 ± 4.815 ng/mL) in normal weight subjects. In obese subjects mean irisin levels (75.76 ± 4.06 ng/mL) were significantly (P<0.0001) elevated at 180 minutes post-kisspeptin when compared with pre-kisspeptin irisin levels (41.28 ± 2.89 ng/mL).
Our findings suggest that kisspeptin may have a novel therapeutic potential to induce irisin release in humans which may have anti-obesity effects.
近期研究表明 kisspeptin 在新陈代谢中发挥作用。然而，将身体脂肪/肌肉组织与调控能量平衡的中枢神经系统联系起来的信号本质仍有待阐明。
本研究旨在探究外周给予 kisspeptin-10 对男性人体鸢尾素释放的影响。
对体重正常（n = 8）和肥胖（n = 8）的男性给予 kisspeptin-10。在注射 kisspeptin 前 30 分钟及注射后 210 分钟内，每隔 30 分钟进行一次连续采血。使用 ELISA 试剂盒检测血浆鸢尾素水平。
给予 kisspeptin-10 对体重正常和肥胖参与者的鸢尾素释放均有显著影响（P < 0.0001）。体重正常的受试者在 210 分钟时的平均鸢尾素水平（96.24 ± 1.351 ng/mL）与注射 kisspeptin 前（59.18 ± 4.815 ng/mL）相比显著升高（P < 0.0001）。在肥胖受试者中，与 kisspeptin 注射前鸢尾素水平（41.28 ± 2.89 ng/mL）相比，注射后 180 分钟时平均鸢尾素水平（75.76 ± 4.06 ng/mL）显著升高（P < 0.0001）。
我们的研究结果表明，kisspeptin 可能具有诱导人体鸢尾素释放的新治疗潜力，这可能具有抗肥胖作用。
